RT Journal Article SR Electronic(1) A1 Wu, Hong A1 Song, Zhijun A1 Hentzer, Morten A1 Andersen, Jens Bo A1 Heydorn, Arne A1 Mathee, Kalai A1 Moser, Claus A1 Eberl, Leo A1 Molin, Søren A1 Høiby, Niels A1 Givskov, Michael YR 2000 T1 Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa JF Microbiology, VO 146 IS 10 SP 2481 OP 2493 DO https://doi.org/10.1099/00221287-146-10-2481 PB Microbiology Society, SN 1465-2080, AB The pathogenesis of Pseudomonas aeruginosa is associated with expression of virulence factors, many of which are controlled by two N-acylhomoserine lactone (AHL)-based quorum-sensing systems. Escherichia coli strains equipped with a luxR-based monitor system expressing green fluorescent protein (GFP) in the presence of exogenous AHL molecules were used to detect the production of AHLs from P. aeruginosa in vivo. Mice were challenged intratracheally with alginate beads containing P. aeruginosa and E. coli and killed on different days after the challenge. By means of confocal scanning laser microscopy, GFP-expressing E. coli bacteria could be detected in the lung tissues, indicating production and excretion of AHL molecules in vivo by the infecting P. aeruginosa. AHL signals were detected mainly in lung tissues exhibiting severe pathological changes. These findings support the view that expression of AHL molecules by P. aeruginosa during infection coincides with its pathogenesis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-146-10-2481