1887

Abstract

The differentiation between subspecies, and the phylogenetic position of clones within clones was determined by analysis of restriction fragment length polymorphisms of rDNA (ribotyping). Seventy-five strains belonging to the four subspecies and 13 enteroinvasive (EIEC) strains were compared with the 72 strains of the ECOR collection, which have been classified into four phylogenetic groups (A, B1, B2 and D). Seventeen Shigella dysenteriae ribotypes, 12 Shigella flexneri ribotypes, 23 Shigella boydii ribotypes, 12 ribotypes and 13 EIEC ribotypes were identified following digestion with and Correspondence analysis of the data showed that serotype 13 strains were distantly related to the other strains, and that and were distinct from and The ribotypes of Shigella and ECOR strains were indistinguishable, and and most strains were closely related to phylogenetic group D, whereas serotype 1 strains belonged to phylogenetic group B1, and strains were evenly distributed between the two groups. The strains were distantly related to group B2, which contains strains frequently implicated in extra-intestinal infections in humans. In contrast, the 13 EIEC strains were more widely distributed between phylogenetic groups B1, A and B2. Thus, there was no primordial species and and EIEC strains are derived from different ancestral strains.

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1998-09-01
2021-10-20
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References

  1. Bingen E., Denamur E., Lambert-Zechovsky N., Aujard Y., Brahimi N., Geslin P., Elion J. 1992; Mother-to-infant vertical transmission and cross-colonization of Streptococcus pyogenes confirmed by DNA restriction fragment length polymorphism analysis.. J Infect Dis 165:147–150
    [Google Scholar]
  2. Bingen E., Picard B., Brahimi N., Mathy S., Desjardins P., Elion J., Denamur E. 1998; Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggets horizontal gene transfer from a predominant pool of highly virulent B2 group strains.. J Infect Dis 177:642–650
    [Google Scholar]
  3. Bohnert M.G., d’Hauteville H.M., Sansonetti P.J. 1988; Detection of enteric pathotypes of Escherichia coli by hybridization using six DNA probes.. Ann Inst Pasteur Microbiol 139:189–202
    [Google Scholar]
  4. Brenner D.-J., Fanning G.R., Skerman F.J., Falko W.S. 1972; Polynucleotide sequence divergence among strains of Escherichia coli and closely related organisms.. J Bacteriol 109:953–965
    [Google Scholar]
  5. Brenner D.J., Fanning G.R., Miklos G.V., Steigerwalt A.G. 1973; Polynucleotide sequence relatedness among Shigella species.. Int J Syst Bacteriol 23:1–7
    [Google Scholar]
  6. Brenner D.J., Steigerwalt A.G., Gail Wathen H., Gross R.J., Rowe B. 1982; Confirmation of aerogenic strains of Shigella boydii 13 and further study of Shigella serotypes by DNA relatedness.. J Clin Microbiol 16:432–436
    [Google Scholar]
  7. Desjardins P., Picard B., Kaltenböck B., Elion J., Denamur E. 1995; Sex in Escherichia coli does not disrupt the clonal structure of the population: evidence from random amplified polymorphic DNA and restriction-fragment-length polymorphism.. J Mol Evol 40:440–448
    [Google Scholar]
  8. Dodd C.E.R., Jones D. 1982; A numerical taxonomic study of the genus Shigella. . J Gen Microbiol 128:1933–1957
    [Google Scholar]
  9. Goullet Ph. 1980; Esterase electrophoretic pattern relatedness between Shigella species and Escherichia coli. . J Gen Microbiol 117:493–500
    [Google Scholar]
  10. Goullet Ph., Picard B. 1986a; Comparative esterase electrophoretic polymorphism of Escherichia coli isolates obtained from animal and human sources.. J Gen Microbiol 132:1843–1851
    [Google Scholar]
  11. Goullet Ph., Picard B. 1986b; Highly pathogenic strains of Escherichia coli revealed by the distinct electrophoretic patterns of carboxylesterase B.. J Gen Microbiol 132:1853–1858
    [Google Scholar]
  12. Goullet Ph., Picard B. 1987; Differentiation of Shigella by esterase electrophoretic polymorphism.. J Gen Microbiol 133:1005–1017
    [Google Scholar]
  13. Goullet Ph., Picard B., Toucas M. 1983; Differenciation du serotype 6 des serotypes 1 and 5 de Shigella flexneri par electrophorese et focalisation isoelectrique de leurs esterases.. Ann Microbiol 134B:269–276
    [Google Scholar]
  14. Greenacre M.J. 1984; Theory and applications of corre-spondences.. London: Academic Press;
    [Google Scholar]
  15. Guttman D.S. 1997; Recombination and clonality in natural populations of Escherichia coli. . Trends Ecol Evol 12:16–22
    [Google Scholar]
  16. Hale T.L. 1991; Genetic basis of virulence in Shigella species.. Microbiol Rev 55:206–224
    [Google Scholar]
  17. Hartl D.L., Dykhuizen D.E. 1984; The population genetics of Escherichia coli. . Annu Rev Genet 18:31–68
    [Google Scholar]
  18. Herzer P.J., Inouye S., Inouye M., Whittam T.S. 1990; Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli. . J Bacterial 172:6175–6181
    [Google Scholar]
  19. Johnson R., Colwell R.R., Sakazaki R., Tamura K. 1975; Numerical taxonomy studies of the Enterobacteriaceae. . Int J Syst Bacterial 25:12–37
    [Google Scholar]
  20. Karaolis D.K.R., Lan R., Reeves P.R. 1994; Sequence variation in Shigella sonnei (Sonnei), a pathogenic clone of Escherichia coli, over four continents and 41 years.. J Clin Microbiol 32:796–802
    [Google Scholar]
  21. Lebart L., Morineau A., Warwick K.M. 1984; Multivariate descriptive analysis and related techniques for large matrices.. New York: Wiley Interscience;
    [Google Scholar]
  22. Marranzano M., Giammanco G., d’Hauteville H., Sansonetti P. 1985; Epidemiological markers of Shigella sonnei infections: R- plasmid fingerprint, phage-typing and biotyping.. Ann Inst Pasteur Microbiol 136:339–345
    [Google Scholar]
  23. Maynard Smith J. 1996; Population genetics: an introduction.. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn. II pp. 2685–2690 Edited by Neidhardt F. C. others Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Ménard R., Dehio C., Sansonetti P. 1996; Bacterial entry into epithelial cells: the paradigm of Shigella. . Trends Microbiol 4:220–226
    [Google Scholar]
  25. Ochman H., Selander R.K. 1984; Standard reference strains of Escherichia coli from natural populations.. J Bacteriol 157:690–693
    [Google Scholar]
  26. Ochman H., Whittam T.S., Caugant D.A., Selander R.K. 1983; Enzyme polymorphism and genetic population structure in Escherichia coli and Shigella. . J Gen Microbiol 129:2715–2726
    [Google Scholar]
  27. Orskov I., Orskov F., Jann B., Jann K. 1977; Serology, chemistry and genetics of O and K antigens of Escherichia coli. . Bacteriol Rev 41:667–710
    [Google Scholar]
  28. Picard-Pasquier N., Ouaghed M., Picard B., Goullet Ph., Krishnamoorthy R. 1989; A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.. Electrophoresis 10:186–189
    [Google Scholar]
  29. Pupo G.M., Karaolis R., Lan R., Reeves P.R. 1997; Evolutionary relationship among pathogenic and non pathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies.. Infect Immun 65:2685–2692
    [Google Scholar]
  30. Sansonetti P.J., d’Hauteville H., Ecobichon C., Poucel C. 1983; Molecular comparison of virulence plasmids in Shigella and enteroinvasive Escherichia coli. . Ann Microbiol 134A:295–318
    [Google Scholar]
  31. Selander R.K., Levin B.R. 1980; Genetic diversity and structure in Escherichia coli populations.. Science 210:545–547
    [Google Scholar]
  32. Sereny B. 1955; Experimental Shigella keratoconjunctivitis. A preliminary report.. Acta Microbiol Acad Sci Hung 2:293–296
    [Google Scholar]
  33. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. J Mol Biol 98:503–517
    [Google Scholar]
  34. Szturm-Rubinstein S. 1964; Répartition géographique des biotypes et lysotypes de 743 souches de S. sonnei. . Ann Inst Pasteur 106:114–122
    [Google Scholar]
  35. Tenehaus M., Young F.W. 1985; An analysis and synthesis of multiple correspondence analysis, optimal scaling, dual scaling, homogeneity analysis, and other methods for quantifying categorical multivariate data.. Psychometrika 50:91–119
    [Google Scholar]
  36. Timakov V.D., Petrovskaya V.G., Bondarenko V.M., Khomenko N.A. 1972; Genetic data concerning Shigella flexneri serotypes 5 and 6.. Int J Syst Bacteriol 22:149–154
    [Google Scholar]
  37. Veron M., Le Minor L. 1975; Nutrition and taxonomy of Enterobacteriaceae and related bacteria. II. General results and classification.. Ann Microbiol 126B:111–124
    [Google Scholar]
  38. Whittam T.S., Ochman H., Selander R.K. 1983; Multilocus genetic structure in natural populations of Escherichia coli. . Proc Natl Acad Sci USA 80:1751–1755
    [Google Scholar]
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