1887

Abstract

The region between (293°) and (289°) of the chromosome has been sequenced within the framework of the subtilis 168 international sequencing programme. A primary analysis of the 42 ORFs identified in this 43 kb region is presented. The region included a high proportion of genes that did not show homology with genes in other bacteria. The identified ORFs showed homology to proteins involved in the transport of metal ions, two-component signal transducers, ATP-binding-cassette-type transporters and a sigma factor.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-6-1593
1998-06-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/6/mic-144-6-1593.html?itemId=/content/journal/micro/10.1099/00221287-144-6-1593&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos, C., Spizizen, J., Altschul, S. F., Gish, W., Miller, W. et al. (1961); Requirements for transformation in Bacillus subtilis.. Journal of Bacteriology, 81,:741–746 1990
    [Google Scholar]
  2. Atkins, J. F., Weiss, R. B., Thompson, S., Gesteland, R. F. (1991); Toward a genetic dissection of the basis of triplet decoding, and its natural subversion: programmed reading frame shifts and hops.. Annual Review Genetics, Vol 45, 25,:201–228
    [Google Scholar]
  3. Axelsson, L., Hoick, A. (1995); The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.. Journal of Bacteriology, 177,:2125–2137
    [Google Scholar]
  4. Blattner, F. R., Plunkett, G., Ill, Bloch, C., A. (1997); & 14 other authors, The complete genome sequence of Escherichia coli K-12.. Science, 277,:1453–1462
    [Google Scholar]
  5. Bult, C. J., White, O., Olsen, G. J. (1996); & 37 other authors, Complete genome sequence of the methanogenic archaeon, Metbanococcus jannascbii.. Science, 273,:1058–1073
    [Google Scholar]
  6. Chen, K. C., Chen, J. S., Johnson, J. L. (1986); Structural features of multiple nifH-like sequences and very biased codon usage in nitrogenase genes of Clostridium pasteurianum.. Journal of Bacteriology, 166,:162–172
    [Google Scholar]
  7. Demerec, M., Adelberg, E. A., Clark, A. J., Hartman, P. E. (1968); Proposal for a uniform nomenclature in bacterial genetics.. Journal of General Microbiology, 50,:1–14
    [Google Scholar]
  8. Fleischmann, R. D., Adams, M. D., White, O. (1995); & 37 other authors, Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.. Science, 269,:496–512
    [Google Scholar]
  9. Fujita, T., Shirasawa, T., Uchida, K., Maruyama N. (1992); Isolation of cDNA clone encoding rat senescence marker protein- 30 (SMP30) and its tissue distribution.. Biochimica Et Biophysica Acta, 1132,:297–305
    [Google Scholar]
  10. Glaser, P., Kunst, F., Arnaud, M. (1993) & 14 other authors, Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325° to 333°.. Molecular Microbiology, 10,:371–384
    [Google Scholar]
  11. Harwood, C. R., Wipat A. (1996); Sequencing and functional analysis of the genome of Bacillus subtilis strain 168.. Febs Letters, 389,:84–87
    [Google Scholar]
  12. Kaneko, T., Tanaka, A., Sato, S., Kotani, H., Sazuka, T. et al. (1995); Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803.1. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome.. Dna Research, 2,:153–166
    [Google Scholar]
  13. Karmazyn-Campelli, C., Bonamy, C., Savelli, B., Stragier, P. (1989); Tandem genes encoding sigma-factors for consecutive steps of development in Bacillus subtilis.. Genes Dev, 3,:150–157
    [Google Scholar]
  14. Kunst, F., Ogasawara, N., Moszer, I. (1997); & 148 other authors, The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.. Nature, 390,:249–256
    [Google Scholar]
  15. Lapidus, A., Galleron, N., Sorokin, A., Ehrlich, D. (1997); Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB—dnaB region.. Microbiology, 143,:3431–3441
    [Google Scholar]
  16. Lee, J. W., Hulett F. M. (1992); Nucleotide sequence of the pboP gene encoding PhoP, the response regulator of the phosphate regulon of Bacillus subtilis.. Nucleic Acids Research, 20,:5848
    [Google Scholar]
  17. Lee, P. J., Stock, A. M. (1996); Characterization of the genes and proteins of a two-component system from the hyperthermophilic bacterium Thermotoga maritima.. Journal of Bacteriology, 178,:5579–5585
    [Google Scholar]
  18. Maupin-Furlow, J. A., Rosentel, J. K., Lee, J. H., Deppenmeier, U., Gunsalus, R. P. et al. (1995); Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli.. Journal of Bacteriology, 177,:4851M856
    [Google Scholar]
  19. Miles, J. S., Guest, J. R. (1985); Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.. Nucleic Acids Research, 13,:131–140
    [Google Scholar]
  20. O'Connell-Motherway, M.„ Fitzgerald, G., F., van Sinderen, D. (1997); Cloning and sequence analysis of histidine protein kinases isolated from Lactococcus lactis MG1363.. Appl Environ Microbiol, 63,:2454–2459
    [Google Scholar]
  21. Ogasawara, N., Nakai, S., Yoshikawa, N. (1994); Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin.. Dna Research, 1,:1–14
    [Google Scholar]
  22. Ohtani, K., Bando, M. (1997); Collagenase gene {colA) is located in the 3'- flanking region of the perfringolysin O (pfoA) locus in Clostridium perfringens.. Fems Microbiology Letters, 146,:155–159
    [Google Scholar]
  23. Sambrook, J., Fritsch, E. F., Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual., 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Schneider, R., Hantke, K. (1993); Iron-hydroxamate uptake systems in Bacillus subtilis: identification of a lipoprotein as part of a binding protein-dependent transport system.. Molecular Microbiology, 8,:111–121
    [Google Scholar]
  25. Schwecke.T., Aparicio, J., F., Molnar, I. (1995); & 10 other authors, The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin.. Proc Natl Acad Sci USA, 92,:7839–7843
    [Google Scholar]
  26. Simonen, M., Palva, I. (1993); Protein secretion in Bacillus species.. Microbiological Reviews, 57,:109–137
    [Google Scholar]
  27. Smith, H., de Jong, A., Bron, S., Venema, G. (1988); Characterization of signal-sequence-coding regions selected from the Bacillus subtilis chromosome.. Gene, 70,:351–361
    [Google Scholar]
  28. Sorokin, A., Zumstein, E., Azevedo, V., Ehrlich, S. D., Serror, P. (1993); The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data.. Molecular Microbiology, 10,:385–395
    [Google Scholar]
  29. Tait-Kamradt, A., Clancy, J., Cronan, M., DibHajj, F., Wondrack, L. et al. (1997); mefE is necessary for the erythromycin-resistant M phenotype in Streptococcus pneumoniae.. Antimicrob Agents Chemother, 41,:2251–2255
    [Google Scholar]
  30. Tanaka, T., Kawata, M. (1988); Cloning and characterization of Bacillus subtilis iep, which has positive and negative effects on production of extracellular proteases.. Journal of Bacteriology, 170,:3593–3600
    [Google Scholar]
  31. Vieira, J., Messing, J. (1987); Production of single-stranded plasmid DNA.. Methods in Enzymology, Vol 46: Research on Nitrification and Related Processes, Pt B, 153,:3–15
    [Google Scholar]
  32. Wipat, A., Carter, N., Brignell, S. C., Guy, B. J., Piper, K. et al. (1996); The dnaB-pheA (256°-240°) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.. Microbiology, 142,:3067–3078
    [Google Scholar]
  33. Yamane, K., Kumano, M., Kurita, K. (1996); The 25°-36° region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes.. Microbiology, 142,:3047–3056
    [Google Scholar]
  34. Ylihonko, K., Tuikkanen, J.„ Jussila, Cong, S. L, et al. (1996); A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.. Molecular & General Genetics, 251,:113–120
    [Google Scholar]
  35. Zuberi, A. R., Moir, A., Feavers, I. M. (1987); The nucleotide sequence and gene organisation of the gerA spore germination operon of Bacillus subtilis 168.. Gene, 51,:1–11
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-6-1593
Loading
/content/journal/micro/10.1099/00221287-144-6-1593
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error