Summary: Bacterial P proteins, encoded by genes, are central signalling molecules in nitrogen regulatory pathways and are modulated by post-translational modification in response to the cellular nitrogen status. The gene was cloned from the filamentous heterocyst-forming cyanobacterium strain ATCC 29133 (PCC 73102) by heterologous hybridization to a sp. strain PCC 7942 gene fragment. Expression of the cloned gene was verified by hybridization to total RNA and a single cross-reactive polypeptide was observed in immunoblots of extracts probed with anti- 7942 P antiserum. Modification of the purified P protein by a 7942 P kinase was observed, but modified forms of P were not detected in extracts of from a variety of incubation conditions. The gene could not be disrupted by targeted gene replacement unless a second copy of was provided suggesting that the gene or gene product is essential for growth under the conditions tested.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error