1887

Abstract

Summary: The hydrophobic probe -phenyl-1-naphthylamine accumulated less in nonpathogenic spp. and non-pathogenic and pathogenic than in or This was largely due to differences in the activity of efflux systems, but also to differences in outer membrane permeability because uptake of the probe in KCN/arsenate-poisoned cells was slower in the former group than in and The probe accumulation rate was higher in and grown at 37 °C than at 26 °C and was always highest in These yersiniae had LPSs with shorter polysaccharides than particularly when grown at 37 °C. Gel liquid-crystalline phase transitions (T 28–31 °C) were observed in LPS aggregates of grown at 26 and 37 °C, with no differences between nonpathogenic and pathogenic strains. and LPSs showed no phase transitions and, although the fluidity of LPSs of and grown at 26 °C were close below the T of the latter, they were always in a more fluid state than LPS. Comparison with previous studies of subsp. serotype minnesota rough LPS showed that the increased fluidity and absence of transition of and LPSs cannot be explained by their shorter polysaccharides and suggested differences at the lipid A/core level. It is proposed that differences in LPS-LPS interactions and efflux activity explain the above observations and reflect the adaptation of spp. to different habitats.

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/content/journal/micro/10.1099/00221287-144-6-1517
1998-06-01
2019-08-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-6-1517
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