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Volume 144, Issue 4

Carbapenems as inhibitors of OXA-13, a novel, integron-encoded β-lactamase in Free

Abstract

A clinical strain, PAe391, was found to be resistant to a number of antibiotics including ticarcllin, piperacillin, cefsulodin and amikacin, and a disk diffusion assay showed evidence of pronounced synergy between imipenem and various β;-lactam antibiotics. Cloning and nucleotide sequence analysis revealed the dicistronic arrangement of an (6’)- variant and a novel -type gene between the and qacEδ1 genes typical of integrons. In PAe391, this integron was apparently chromosome-borne. The β;-lactamase, named OXA-13, displayed nine amino acid changes with respect to OXA-10: I in position 10 of OXA-10 to T (I10T), G20S, D55N, N73S, T107S, Y174F, E229G, S245N and E259A. OXA-13 (pl = 8.0) showed poor catalytic activity against penicillins as well as cephalosporins, but was efficient in hydrolysing some penicillinase-resistant β;-lactams, such as cefotaxime and aztreonam. It was efficiently inhibited by imipenem ( = 11 nM), and formed a stable complex. While the value of meropenem was similar (16 nM), the corresponding complex was less stable.

  • Received:
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Keyword(s): carbapenem , integron , oxacillinase , Pseudomonas aeruginosa and β-lactam resistance
© Society for General Microbiology 1998
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1998-04-01
2023-12-04
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Carbapenems as inhibitors of OXA-13, a novel, integron-encoded β-lactamase in Pseudomonas aeruginosa
Microbiology 144, 1021 (1998); https://doi.org/10.1099/00221287-144-4-1021
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