1887

Abstract

SUMMARY: Secreted aspartic proteinases (Saps) contribute to the virulence of Candida albicans in systemic animal models of infection. Seven genes encoding Saps (SAM-SAP7) have been identified to date but evidence suggested the existence of additional SAP genes. The screening of a C. albicans iZEMBL3 genomic library for the presence of other SAP genes was undertaken. Two new genes, SAP8 and SAPS, were isolated. The N-terminal amino acid sequence deduced from SAP8 downstream of a Kex2plike cleavage site corresponds to the N-terminal amino acid sequence of the 41 kDa Sap isolated and characterized previously. SAP8 mRNA was expressed preferentially in yeasts at 25 "C after 6 and 9 h growth in BSA-containing medium. SAPS encodes an aspartic proteinase with a Kex2pllike cleavage site and contains a putative glycophosphatidylinositol-anchor signal at the C-terminus. Although the SAPS gene product has not yet been isolated from cultures of C. albicans, transcripts of SAPS were observed preferentially in later growth phases when SAP8 expression had decreased.

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1998-10-01
2021-10-20
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References

  1. Ash J., Dominguez M., Bergeron J.J.M., Thomas D.Y., Bourbonnais Y. 1995; The yeast proprotein convertase encoded by YAP3 is a glycophosphatidylinositol-anchored protein that localizes to the plasma membrane.. J Biol Chem 270:20847–20854
    [Google Scholar]
  2. Chambers S.P., Prior S.E., Barstow D.A., Milton N.P. 1988; The pMTL nic-cloning vectors. 1. Improved poly linker region to facilitate the use of sonicated DNA for nucleotide sequencing.. Gene 68:139–149
    [Google Scholar]
  3. Colthurst D.R., Schauer B.S., Hates M.V., Tuite M.F. 1992; Elongation factor 3 (EF-3) from Candida albicans shows both structural and functional similarity to EF-3 from Saccharomyces cerevisiae. . Mol Microbiol 6:1025–1033
    [Google Scholar]
  4. Crandall M., Edwards J.E. 1987; Segregation of proteinasenegative mutants from heterozygous Candida albicans. . J Gen Microbiol 133:2817–2824
    [Google Scholar]
  5. Egel-Mitani M., Flygenring H.P., Hansen M.T. 1990; A novel aspartyl protease allowing KEX2-independent MFα prophero-mone processing in yeast.. Yeast 6:127–137
    [Google Scholar]
  6. Gerber L.D., Kodukula K., Udenfriend S. 1992; Phospha- tidylinositol glycan (PI-G) anchored membrane proteins.. J Biol Cbem 267:12168–12173
    [Google Scholar]
  7. Grossberger D. 1987; Minipreps of DNA from bacteriophage lambda.. Nucleic Acids Res 15:6737
    [Google Scholar]
  8. von Heijne G. 1986; A new method for predicting signal sequence cleavage sites.. Nucleic Acids Res 14:4683–4690
    [Google Scholar]
  9. Higgins D.G., Sharp P.M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer.. CABIOS 5:151–153
    [Google Scholar]
  10. Hube B., Monod M., Schofield A., Brown A.J.P., Gow N.A.R. 1994; Expression of seven members of the gene family encoding secretory aspartyl proteinases in Candida albicans. . Mol Microbiol 14:87–99
    [Google Scholar]
  11. Hube B., Sanglard D., Monod M., Brown A.J.P., Gow N.A.R. 1997a; Extracellular proteolytic activity of Candida species. In Host-Fungus Interplay. . Proceedings of the 5th Symposium on Topics in Mycology 1995 pp. 109–122 Edited by Vanden Bossche H., Stevens D. A., Odds F. C. Bethesda, MD: National Foundation for Infectious Diseases;
    [Google Scholar]
  12. Hube B., Sanglard D., Odds F. C., Hess D., Monod M., Schafer W., Brown A.J.P., Gow N.A.R. 1997b; Disruption of each of the aspartyl proteinase genes SAP1, SAP2, and SAP3 of Candida albicans attenuates virulence.. Infect lmmun 65:3529–3538
    [Google Scholar]
  13. Julius D., Brake A., Blair L., Kunisawa R., Thorner J. 1984; Isolation of the putative structural gene for the lysine-argininecleaving endopeptidase required for processing of yeast prepro-α- factor.. Cell 37:1075–1089
    [Google Scholar]
  14. Monod M., Togni G., Hube B., Sanglard D. 1994; Multiplicity of genes encoding secreted aspartic proteases in Candida species.. Mol Microbiol 13:357–368
    [Google Scholar]
  15. Morrison C.J., Hurst S.F., Bragg S.L., Kuykendall R.J., Diaz H., Pohl J., Reiss E. 1993; Heterogeneity of the purified extracellular aspartyl proteinase from Candida albicans-, characterization with monoclonal antibodies and N-terminal amino acid sequence analysis.. Infect lmmun 61:2030–2036
    [Google Scholar]
  16. Morrow B., Srikantha T., Soil D.R. 1992; Transcription of the gene for a pepsinogen, PEP1, is regulated by white-opaque switching in Candida albicans. . Mol Cell Biol 12:2997–3005
    [Google Scholar]
  17. Morrow B., Ramsey H., Soil D.R. 1994; Regulation of phase- specific genes in the more general switching system of Candida albicans strain 3153A.. J Med Vet Mycol 32:287–294
    [Google Scholar]
  18. Newport G., Agabian N. 1997; KEX2 influences Candida albicans proteinase secretion and hyphal formation.. J Biol Chem 272:28954–28961
    [Google Scholar]
  19. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Sanglard D., Hube B., Monod M., Odds F.C., Gow N.A.R. 1997; A triple deletion of the aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence.. Infect lmmun 65:3539–3546
    [Google Scholar]
  21. Soll D.R. 1992; High-frequency switching in Candida albicans. . Clin Microbiol Rev 5:183–185
    [Google Scholar]
  22. Swoboda R.K., Bertram G., Colthurst D.R., Tuite M.F., Gow N.A.R., Gooday G.W., Brown A.J.P. 1994; Regulation of the gene encoding translation elongation factor 3 during growth and morphogenesis in Candida albicans. . Microbiology 140:2611–2616
    [Google Scholar]
  23. Togni G., Sanglard D., Falchetto R., Monod M. 1991; Isolation and nucleotide sequence of the extracellular acid protease gene (ACP) from the yeast Candida tropicalis. . FEBS Lett 286:181–185
    [Google Scholar]
  24. de Viragh P.A., Sanglard D., Togni G., Falchetto R., Monod M. 1993; Cloning and sequencing of two Candida parapsilosis genes encoding acid proteases.. J Gen Microbiol 139:335–342
    [Google Scholar]
  25. White T.C., Agabian N. 1995; Candida albicans secreted aspartyl proteinases: isoenzyme pattern is determined by cell type, and levels are determined by environmental factors.. J Bacteriol 177:5215–5221
    [Google Scholar]
  26. White T.C., Miyasaki S.H., Agabian N. 1993; Three distinct secreted aspartyl proteinases in Candida albicans. . J Bacteriol 175:6126–6133
    [Google Scholar]
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