The gene from was cloned using degenerate oligonucleotide primers designed from conserved regions of RecA proteins from other bacteria. The 1089 bp gene encoded a putative RecA protein with 69% amino acid sequence similarity to the RecA protein from . The gene was induced by exposure to methyl methanesulphonate and complemented a mutant of . A Cheo box was identified in the region upstream of the gene. Since this SOS-like operator site is conserved in many DNA-damage-inducible gene regions from Gram-positive bacteria, the results suggest that the regulation of the gene also involves the binding of a LexA-like protein to this site.


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