%0 Journal Article %A Johnston, Joanne L. %A Sloan, Joan %A Fyfe, Janet A. M. %A Davies, John K. %A Rood, Julian I. %T The recA gene from Clostridium perfringens is induced by methyl methanesulphonate and contains an upstream Cheo box %D 1997 %J Microbiology, %V 143 %N 3 %P 885-890 %@ 1465-2080 %R https://doi.org/10.1099/00221287-143-3-885 %K Bacillus subtilis %K RecA %K SOS operator site %K regulation %K DNA damage %K Cheo box %I Microbiology Society, %X The recA gene from Clostridium perfringens was cloned using degenerate oligonucleotide primers designed from conserved regions of RecA proteins from other bacteria. The 1089 bp gene encoded a putative RecA protein with 69% amino acid sequence similarity to the RecA protein from Bacillus subtilis. The C. perfringens recA gene was induced by exposure to methyl methanesulphonate and complemented a recA mutant of Escherichia coli. A Cheo box was identified in the region upstream of the gene. Since this SOS-like operator site is conserved in many DNA-damage-inducible recA gene regions from Gram-positive bacteria, the results suggest that the regulation of the C. perfringens recA gene also involves the binding of a LexA-like protein to this site. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-3-885