@article{mbs:/content/journal/micro/10.1099/00221287-143-3-885, author = "Johnston, Joanne L. and Sloan, Joan and Fyfe, Janet A. M. and Davies, John K. and Rood, Julian I.", title = "The recA gene from Clostridium perfringens is induced by methyl methanesulphonate and contains an upstream Cheo box", journal= "Microbiology", year = "1997", volume = "143", number = "3", pages = "885-890", doi = "https://doi.org/10.1099/00221287-143-3-885", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-3-885", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Bacillus subtilis", keywords = "RecA", keywords = "SOS operator site", keywords = "regulation", keywords = "DNA damage", keywords = "Cheo box", abstract = "The recA gene from Clostridium perfringens was cloned using degenerate oligonucleotide primers designed from conserved regions of RecA proteins from other bacteria. The 1089 bp gene encoded a putative RecA protein with 69% amino acid sequence similarity to the RecA protein from Bacillus subtilis. The C. perfringens recA gene was induced by exposure to methyl methanesulphonate and complemented a recA mutant of Escherichia coli. A Cheo box was identified in the region upstream of the gene. Since this SOS-like operator site is conserved in many DNA-damage-inducible recA gene regions from Gram-positive bacteria, the results suggest that the regulation of the C. perfringens recA gene also involves the binding of a LexA-like protein to this site.", }