1887

Abstract

Two isogenic strains of K-12 differing only in , as well as two transductants of the strain, were examined with respect to ppGpp levels and reversion rates of a allele under nine different conditions. A positive correlation was established between reversion rates and the steady-state concentration of ppGpp during exponential growth. The genes from two strains (isogenic except for ) were cloned and sequenced and found to contain a single mutation, namely, a C-to-T transition at nucleotide 857. This mutation resulted in a serine-to-leucine substitution at amino acid residue 286 of the LeuB protein. PCR products that encompassed the lesion were generated from 53 revertants and then sequenced. Of these revertants, 36 were found to contain nucleotide substitutions that would result in a serine (wild type), valine or methionine at amino acid residue 286 of LeuB, and nearly all of them exhibited generation times similar to wild type. Seventeen of the analysed revertants were found to be suppressors that retained the encoded leucine at residue 286. The majority of the suppressor mutants exhibited generation times that were significantly longer than wild type.

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1997-03-01
2021-07-23
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