1887

Abstract

Using the operon fusion technique, the transcriptional control of the gene was studied. A low (approximately twofold) inductive capacity was observed for compounds that damage DNA and/or inhibit DNA replication, e.g. methyl methanesulfonate, mitomycin C, UV light and nalidixic acid. Induction of the gene by DNA damage was independent of functional RecA. The presence of the promoter region on a multicopy plasmid had the same effect on transcription as the presence of DNA-damaging agents. Thus, expression in appears to be regulated in a novel fashion, possibly involving a non-LexA-like repressor. Regulation of the gene in appears not to be part of a regulon responsible for competence for natural transformation: in cells exhibiting extremely low transformation frequencies, the level of transcription of the gene was found to be comparable to the level found in cells in the state of maximal competence.

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1996-04-01
2021-05-12
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