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Abstract
The activity of BstVI DNA methyltransferase was monitored during the sporulative cycle of Bacillus stearothermophi lus V. Significant methylase activity was found only in bacteria growing vegetatively. This was confirmed by Northern hybridization, which indicated that the bstVIM gene was not transcribed in cells undergoing sporulation. Supporting evidence came from experiments which demonstrated that the RNA polymerase holoenzyme from these cells did not recognize the promoter elements upstream of the bstVIM gene.
- Received:
- Accepted:
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- Published Online:
Keyword(s):
Bacillus stearothermophilus
,
differential gene expression
,
sporulation
and
thermophilic promoters
© Society for General Microbiology 1994