1887

Abstract

Summary: The gene for the periplasmic β-lactamase of was isolated as part of a 1017 bp RI fragment and the nucleotide sequence of the gene was determined. It has a G + C content of 71.5% and encodes a 27 amino acid signal sequence and the mature β-lactamase of 276 amino acids which has a mass of 29146 Da. The enzyme appears to be unique to but its amino acid sequence shows a high degree of homology with the amino acid sequences of the lactamase from and other Class A β-lactamases. The β-lactamase gene of was expressed in using pUC118 as the vector. The production of active β-lactamase was highest after the active growth phase of the expression host and reached levels which were about three times higher than those obtained with

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/content/journal/micro/10.1099/00221287-139-6-1245
1993-06-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1245
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