1887

Abstract

The gene for the periplasmic -lactamase of was isolated as part of a 1017 bp RI fragment and the nucleotide sequence of the gene was determined. It has a G + C content of 71·5% and encodes a 27 amino acid signal sequence and the mature -lactamase of 276 amino acids which has a mass of 29146 Da. The enzyme appears to be unique to but its amino acid sequence shows a high degree of homology with the amino acid sequences of the lactamase from and other Class A -lactamases. The -lactamase gene of was expressed in using pUC118 as the vector. The production of active -lactamase was highest after the active growth phase of the expression host and reached levels which were about three times higher than those obtained with

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1993-06-01
2021-07-24
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