1887

Abstract

Summary: A study was made of the antagonistic effect of on the cellulolytic activity of . An extracellular factor inhibiting the cellulolytic activity of the fungus was detected in the bacterial supernatant. The antagonistic factor, which precipitated with ammonium sulphate at 40% saturation, was temperature-sensitive and was destroyed at temperatures above 60 °C. After separation by anion-exchange chromatography, sequential precipitation, dialysis and SDS-PAGE, two protein species of 100 and 24 kDa were identified as being involved in this antagonistic effect. It is not known whether the proteins are two subunits of a single protein or represent two different proteins. The inhibitory factor, which is not a bacterial cellulase, did not affect fungal growth, but it inhibited the activity of the fungal cellulases.

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/content/journal/micro/10.1099/00221287-139-4-873
1993-04-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-4-873
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