RT Journal Article SR Electronic(1) A1 Bernalier, Annick A1 Fonty, Gérard A1 Bonnemoy, Frédérique A1 Gouet, PhilippeYR 1993 T1 Inhibition of the cellulolytic activity of Neocallimastix frontalis by Ruminococcus flavefaciens JF Microbiology, VO 139 IS 4 SP 873 OP 880 DO https://doi.org/10.1099/00221287-139-4-873 PB Microbiology Society, SN 1465-2080, AB A study was made of the antagonistic effect of Ruminococcus flavefaciens on the cellulolytic activity of Neocallimastix frontalis. An extracellular factor inhibiting the cellulolytic activity of the fungus was detected in the bacterial supernatant. The antagonistic factor, which precipitated with ammonium sulphate at 40% saturation, was temperature-sensitive and was destroyed at temperatures above 60 °C. After separation by anion-exchange chromatography, sequential precipitation, dialysis and SDS-PAGE, two protein species of 100 and 24 kDa were identified as being involved in this antagonistic effect. It is not known whether the proteins are two subunits of a single protein or represent two different proteins. The inhibitory factor, which is not a bacterial cellulase, did not affect fungal growth, but it inhibited the activity of the fungal cellulases., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-4-873