1887

Abstract

Two components of the maltose transport system, maltoporin (OmpM) and an osmotically shockable periplasmic maltose-binding protein (MBP) were identified. The synthesis of OmpM (apparent 43000) and transport of maltose into cells of were induced by maltose and maltodextrins. A mutant lacking OmpM was drastically impaired in maltose transport, independent of induction by maltose. The MBP of (apparent 40000) was found in the osmotic shock fluid. Its synthesis was induced by maltose. Moreover, rabbit antibodies raised against the MBP of cross-reacted with the analogous protein from . The MBP of restored the maltose transport activities in Δ mutant cells of

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1993-02-01
2021-09-24
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References

  1. Ahmad S., Weisburg W.G., Jensen R.A. 1990; Evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria. Journal of Bacteriology 170:5916–5918
    [Google Scholar]
  2. Ames G.F.-L., Prody C, Kustu S. 1984; Simple, rapid, and quantitative release of periplasmic proteins by chloroform. Journal of Bacteriology 160:1181–1183
    [Google Scholar]
  3. Bielecki J., Hrebenda J. 1988; Outer membrane protein ofYersinia:majorprotein induced by maltose. Acta Microbiologica Polonica 37:133–139
    [Google Scholar]
  4. Brass J.M., Manson M.D. 1984; Reconstitution of maltose chemotaxis in Escherichia coli by addition of maltose binding protein to calcium-treated cells of maltose regulon mutants. Journal of Bacteriology 157:881–890
    [Google Scholar]
  5. Brass J.M., Ehmann V., Bukau B. 1983; Reconstitution of maltose transport in Escherichia coli: conditions affecting import of maltose binding protein into periplasm of calcium treated cells. Journal of Bacteriology 155:76–106
    [Google Scholar]
  6. Brenner D.J. 1984; Facultatively anaerobic Gram-negative rods.Family I, Enterobacteriaceae . 1 pp. 408–420 Krieg N.R., Holt J.G. Edited by In Bergey’s Manual of Systematic Bacteriology, Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Brzostek K., Hrebenda J. 1988; Outer-membrane permeability to β-lactam antibiotics in Yersinia enterocolitica. Journal of General Microbiology 134:1535–1540
    [Google Scholar]
  8. Brzostek K., Nichols W.W. 1990; Outer membrane permeability and porin proteins of Yersinia enterocolitica. FEMS Microbiology Letters 70:275–278
    [Google Scholar]
  9. Brzostek K., Hrebenda J., Benz R., Boos W. 1989; The OmpC protein of Yersinia enterocolitica :purification and properties. Research in Microbiology 140:599–614
    [Google Scholar]
  10. Carlton B.C., Brown B.J. 1981; Gene mutation. pp. 222–242 Gerhardt P.R.G.E., Murray R.G.E, Costilow R.N, Nester E.W., Wood W.A., Krieg N.R., Philips G.B. Edited by In Manual of Methods for General Bacteriology, Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Casadaban M.J. 1976; Transposition and fusion of the lac genesto selected promoters in Escherichia coli using bacteriophage lambda and mu. Journal of Molecular Biology 104:541–555
    [Google Scholar]
  12. Diedrich D.L., Fralick J.A. 1982; Relationship between the OmpC and LamB proteins of Escherichia coli and its influence on the protein mass of theouter membrane. Journal of Bacteriology 149:156–160
    [Google Scholar]
  13. Hengge R., Boos W. 1983; Maltose and lactose transport in Escherichia coli. Examples of two different types of concentrative transport system. Biochimica et Biophysica Acta 737:443–478
    [Google Scholar]
  14. Henning U., Sontag I., Hindennach I. 1978; Mutants (OmpA) affecting a major outer membrane protein of Escherichia coli K-12. European Journal of Biochemistry 92:491–498
    [Google Scholar]
  15. Heppel L.A. 1971; The concept of periplasmic enzymes. pp. 223–247 Rothfield L.I. Edited by In Structure and Function of Biological Membranes New York: Academic Press;
    [Google Scholar]
  16. Hirvas L., Koski P., Vaara M. 1991; The ompH gene of Yersinia enterocolitica: cloning, sequencing, expression, and comparison with known enterobacterial ompH sequences. Journal of Bacteriology 173:1223–1229
    [Google Scholar]
  17. Hofstra H., Van Tol M.J.D., Vaara M. 1980; Cross-reactivity of major outer membrane proteins of Enterobacteriaceae, studied by crossed immunoelectrophoresis. Journal of Bacteriology 143:328–337
    [Google Scholar]
  18. Kellerman O., Szmelcman S. 1974; Active transport of maltose in Escherichia coli K-12.Involvement of a “periplasmic” maltose binding protein. European Journal of Biochemistry 86:27–34
    [Google Scholar]
  19. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  20. Lugtenberg B. 1981; Composition and function of the outer membrane of Escherichia coli. Trends in Biochemical Sciences 6:262–266
    [Google Scholar]
  21. Lugtenberg B., Meijers J., Peters R., Van Der Hoek P., Van Alphen L. 1975; Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K-12 into four bands. FEBS Letters 58:254–258
    [Google Scholar]
  22. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor; NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  23. Nakae T. 1976; Identification of the major outer membrane protein of Escherichia coli that produces transmembrane channels in reconstituted vesicle membranes Biochemical and Biophysical Research Communications . 71:877–884
    [Google Scholar]
  24. Nikaido H., Vaara M. 1985; Molecular basis of bacterial outer membrane permeability. Microbiological Reviews 49:1–32
    [Google Scholar]
  25. Palva A. 1983; OmpA gene in the detection of Escherichia coli and other Enterobacteriaceae by nucleic acid sandwich hybridization. Journal of Clinical Microbiology 18:92–100
    [Google Scholar]
  26. Palva E.T., Westerman P. 1979; Arrangement of the maltose-inducible major outer membrane proteins, the bacteriophage λ receptor in Escherichia coli and the 44K protein in Salmonella typhimurium. FEBS Letters 95:77–80
    [Google Scholar]
  27. Pick K.H., WöBer G. 1979; Maltodextrin pore proteins in the outer membrane of Escherichia coli and Klebsiella pneumoniae: immunological comparison. FEMS Microbiological Letters 5:119–122
    [Google Scholar]
  28. Schuman J. 1982; Active transport of maltose in Escherichia coli K-12.Role of the periplasmic maltose binding protein and evidence for a substrate recognition site in the cytoplasmic membrane. Journal of Biological Chemistry 257:5455–5461
    [Google Scholar]
  29. Schwartz M. 1987; The maltose regulon.. In Escherichia coli and Salmonella typhimurium pp. 1482–1502 Ingraham J.L., Low K.B., Magasanik B., Umbarger H.E. Edited by Cellular and Molecular Biology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. Szmelcman S., Hofnung M. 1975; Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor. Journal of Bacteriology 124:112–118
    [Google Scholar]
  31. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  32. Treptow N.A., Shuman H.A. 1985; Genetic evidence for substrate and periplasmic-binding-protein recognition by the MalF and MalG protein, cytoplasmic membrane component of the Escherichia coli maltose transport system. Journal of Bacteriology 163:654–660
    [Google Scholar]
  33. Venegas A., Gomez I., Zaror I., Yudelevich A. 1988; The nucleotide sequence of the Salmonella typhi ompC porin gene. Nucleic Acids Research 16:7721–7722
    [Google Scholar]
  34. Wandersman C., Schwartz M., Ferenci T. 1979; Escherichia coli mutants impaired in maltodextrin transport. Journal of Bacteriology 140:1–13
    [Google Scholar]
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