1887

Abstract

The yeast-mycelium dimorphism of was studied in continuous culture in a defined medium. At a constant dilution rate (0·08 h) the morphological status of the culture could be controlled by the input concentration of Zn. As the input concentration of Zn was increased (in intervals from 0 to 7·6 μ) the culture shifted from a zinc-limited to a carbon-limited state. In this interval the culture gradually passed through three growth regimes based on morphology and concentration of exopolysaccharide and biomass. The first growth regime was found when the input concentration of Zn was kept below 0·45 μ. Growth in this regime was zinc-limited and more than 90% of the biomass was in the yeast growth form. An increase in the input concentration of Zn in this growth regime led to a proportional increase in both the biomass and the concentration of exopolysaccharide. When the input concentration of Zn was varied between 0·45 μ and 0·80 μ a second growth regime could be detected where simultaneous limitations in two nutrients were recognized. Although the carbon source (glucose) was exhausted an increase in the input concentration of Zn led to a proportional increase in the steady-state biomass concentration. The increase in biomass concentration was at the expense of exopolysaccharide production, which gradually decreased. The culture, still being primarily limited by Zn, remained in the yeast growth form. In a third growth regime (input concentration of Zn above 0·80 μ) no increase in the steady-state biomass was seen when the input concentration of Zn was increased. The concentration of residual glucose and exopolysaccharide was close to zero, and no further carbon could be diverted to an increase in the biomass. Glucose was the primary limiting nutrient. Increasing the input concentration of Zn in this growth regime led to a gradual increase in the mycelial growth form at the expense of the yeast growth form. More than 90% of the biomass was in the mycelial growth form when an input concentration of Zn of 7·6 μ was used.

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1993-12-01
2024-12-08
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References

  1. Alsina A., Rodriguez-Del Valle N. 1984; Effects of divalent cations and functionally related substances on the yeast to mycelium transition in Sporothrix schenckii. Sabouraudia 22:1–5
    [Google Scholar]
  2. Bartnicki-Garcia S., Nickerson W. J. 1962; Nutrition, growth and morphogenesis of Mucor rouxii. Journal of Bacteriology 84:841–858
    [Google Scholar]
  3. Bedell G.W., Soll D.R. 1979; Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: Evidence for zinc-resistant and -sensitive pathways for mycelium formation. Infection and Immunity 26:348–354
    [Google Scholar]
  4. Catley B.J. 1973; The rate of elaboration of the extracellular polysaccharide, pullulan, during growth of Pullularia pullulans. Journal of General Microbiology 78:33–38
    [Google Scholar]
  5. Catley B.J. 1979; Pullulan synthesis by Aureobasidium pullulans. In Microbial Polysaccharides and Polysaccharases pp. 69–84 Edited by Berkeley R. C. W., Gooday G. W., Ellwood D. C. London: Academic Press;
    [Google Scholar]
  6. Catley B.J. 1980; The extracellular polysaccharide, pullulan, produced by Aureobasidium pullulans: a relationship between elaboration rate and morphology. Journal of General Microbiology 120:265–268
    [Google Scholar]
  7. Catley B. J. 1979; Pullulan synthesis by Aureobasidium pulluluns. In Microbial Polysaccharides and Polysaccharases pp. 69–84 Edited by Berkeley R. C. W., Gooday G. W., Ellwood D. C. London: Academic Press;
    [Google Scholar]
  8. Catley B.J. 1980; The extracellular polysaccharide, pullulan, produced by Aureobasidium pullulans: a relationship between elaboration rate and morphology. Journal of General Microbiology 120:265–268
    [Google Scholar]
  9. Duchars M.G., Attwood M.M. 1989; The influence of C:N ratio in the growth medium on the cellular composition and regulation of enzyme activity in Hyphomicrobium X. Journal of General Microbiology 135:787–793
    [Google Scholar]
  10. Egli T., Quayle J.R. 1986; Influence of the carbon: nitrogen ratio of the growth medium on the cellular composition and the ability of the methylotrophic yeast Hansenula polymorpha to utilize mixed carbon sources. Journal of General Microbiology 132:1779–1788
    [Google Scholar]
  11. Gadd G. M., White C., Mowll J.L. 1987; Heavy metal uptake by intact cells and protoplasts of Aureobasidium pullulans. FEMS Microbiology Ecology 45:261–267
    [Google Scholar]
  12. Heald P.J., Kristiansen B. 1985; Synthesis of polysaccharide by yeast-like forms of Aureobasidium pullulans. Biotechnology and Bioengineering 27:1516–1519
    [Google Scholar]
  13. Herbert D., Elsworth R., Telling R.C. 1956; The continuous culture of bacteria; a theoretical and experimental study. Journal of General Microbiology 14:601–622
    [Google Scholar]
  14. Lawford H.G., Pik J.R., Lawford G.R., Williams T., Kligerman A. 1980; Physiology of Candida utilis yeast in zinc- limited chemostat culture. Canadian Journal of Microbiology 26:64–70
    [Google Scholar]
  15. Light P.A. 1972; Influence of environment on mitochondrial function in yeast. Journal of Applied Chemistry and Biotechnology 22:509–526
    [Google Scholar]
  16. Mcneil B., Kristiansen B., Seviour R.J. 1989; Polysaccharide production and morphology of Aureobasidium pullulans in continuous culture. Biotechnology and Bioengineering 33:1210–1212
    [Google Scholar]
  17. Pine L., Peacock C. L. 1958; Studies on the growth of Histoplasma capsulatum. Journal of Bacteriology 75:167–174
    [Google Scholar]
  18. Reeslev M., Nielsen J.C., Jørgensen B.B. 1990; Nutritional dependent dimorphism in the exopolysaccharide producing deutero- mycete Aureobasidium pullulans. Proceedings of the 5th European Congress on Biotechnology 2 pp. 1053–1056
    [Google Scholar]
  19. Reeslev M., Nielsen J.C., Olsen J., Jensen B., Jacobsen T. 1991; Effect of pH and the initial concentration of yeast extract on regulation of dimorphism and exopolysaccharide formation of Aureobasidium pullulans in batch culture. Mycological Research 95:220–226
    [Google Scholar]
  20. Reeslev M. 1992 Exopolysaccharide production, morphogenesis, and viscosity in submerged cultures of Aureobasidium pullulans PhD thesis The Technical University of Denmark; Lyngby, Denmark:
    [Google Scholar]
  21. Roessler W.G., Herbst E.J., Mccullogh W.G., Mills R.C., Brewer C.R. 1946; Studies with Coccidioides immitis. 1. Submerged growth in liquid culture. Journal of Infectious Diseases 79:12–22
    [Google Scholar]
  22. Sabie F.T., Gadd G.M. 1990; Effect of zinc on the yeast- mycelium transition of Candida albicans and examination of zinc uptake at different stages of growth. Mycological Research 94:952–958
    [Google Scholar]
  23. Seviour R.J., Stasinopoulos S.J., Auer D.P.F., Gibbs P.A. 1992; Production of pullulan and other exopolysaccharides by filamentous fungi. Critical Reviews in Biotechnology 12:279–298
    [Google Scholar]
  24. Shepherd M.G., Sullivan P.A. 1976; The production and growth characteristics of yeast and mycelial forms of Candida albicans in continuous culture. Journal of General Microbiology 93:361–370
    [Google Scholar]
  25. Soll D.R., Bedell G.W., Brummel M. 1981; Zinc and the regulation of growth and phenotype in the infectious yeast Candida albicans. Infection and Immunity 32:1139–1147
    [Google Scholar]
  26. Tempest D.W. 1970; The place of continuous culture in microbial research. Advances in Microbial Physiology 4:223–250
    [Google Scholar]
  27. Vallee B.L. 1983; Zinc in biology and biochemistry. In Zinc Enzymes pp. 3–23 Edited by Spiro T. G. New York: John Wiley & Sons;
    [Google Scholar]
  28. Widra A. 1964; Phosphate directed Y-M variation in Candida albicans. Mycopathologia et Mycologia Applicata 23:197–202
    [Google Scholar]
  29. Yamaguchi H. 1975; Control of dimorphism in Candida albicans by zinc: effect on cell morphology and composition. Journal of General Microbiology 86:370–372
    [Google Scholar]
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