1887

Abstract

The attachment site () of phage øU, a lysogenic phage of biovar , was identified on a 6.0 kb fragment of the phage DNA. Plasmid pCI6 was constructed by cloning this fragment into the site of suicide plasmid vector pSUP202. S17-1 harbouring plasmid pCI6 was mated with wild-type biovar strain 4S and its lysogenic strain 4S(øU) using tetracycline resistance (Tc) as a selection marker. The Tc R.leguminosarum biovar transconjugants appeared at high frequency (10-10 per recipient cell in both matings). Southern hybridization with the fragment and pSUP202 as probes indicated that plasmid pCI6 integrated into the chromosome of all these transconjugants in the same manner as phage øU.

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1993-10-01
2021-07-31
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References

  1. Beringer J.E. 1974; R-factor transfer in Rhizobium leguminosarum.. Journal of General Microbiology 84:188–198
    [Google Scholar]
  2. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Buchanan-Wollaston A.V. 1979; Generalized transduction in Rhizobium leguminosarum.. Journal of General Microbiology 112:135–142
    [Google Scholar]
  4. Casse F., Boucher C., Julliot J.S., Michel H., DÉnariÉ J. 1979; Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis.. Journal of General Microbiology 113:229–242
    [Google Scholar]
  5. Dallmann G., Orosz L., Sain B. 1979; Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: comparison of genetic and physical maps indicates a long, genetically silent chromosomal arm.. Molecular and General Genetics 176:439–448
    [Google Scholar]
  6. Donnelly D.F., Birkenhead K., O’gara F. 1987; Stability of IncQ and IncP-1 vector plasmids in Rhizobium spp.. FEMS Microbiology letters 42:141–145
    [Google Scholar]
  7. Dorgai L., Olasz F., BerÉnyi M., Dallmann G., PÁy A., Orosz L. 1981; Orientation of the genetic and physical map of Rhizobium meliloti temperate phage 16-3.. Molecular and General Genetics 182:321–325
    [Google Scholar]
  8. Dorgai L., Olasz F., NÉmeth K. 1986; Lysogenic control of temperate phage 16-3 of Rhizobium meliloti 41 is governed by two distinct regions.. Molecular and General Genetics 205:568–571
    [Google Scholar]
  9. Duchrow M., Giffhorn F. 1987; Physical map of the Rhodobacter sphaeroides bacteriophage ɸRsGl genome and location of the prophage on the host chromosome.. Journal of Bacteriology 169:4410–4414
    [Google Scholar]
  10. Finan T.M., Hartwieg E., Lemieux K., Bergman K., Walker G.C., Signer E.R. 1984; General transduction in Rhizobium meliloti.. Journal of Bacteriology 159:120–124
    [Google Scholar]
  11. Hattori M., Hidaka S., Sakaki Y. 1985; Sequence analysis of a Kpnl family member near the 3´ end of human /β-globin gene.. Nucleic Acids Research 13:7813–7827
    [Google Scholar]
  12. Hermesz E., Olasz F., Dorgai L., Orosz L. 1992; Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system.. Gene 119:9–15
    [Google Scholar]
  13. Higashi S., Abe M. 1980; Promotion of infection thread formation by substances from Rhizobium trifolii.. Journal of Applied and Environmental Microbiology 39:297–301
    [Google Scholar]
  14. Higashi S., Uchiumi T., Abe M. 1983; Elimination of Rhizobium infectivity by temperature treatment.. Journal of General and Applied Microbiology 29:281–285
    [Google Scholar]
  15. Ikeda H., Tomizawa J. 1968; Prophage PI, an extrachromosomal replication unit.. Cold Spring Harbor Symposia on Quantitative Biology 33:791
    [Google Scholar]
  16. Keele B.B., Hamilton P.B., Elkan G.H. 1969; Glucose catabolism in Rhizobium japonicum.. Journal of Bacteriology 97:1184–1191
    [Google Scholar]
  17. Kowalski M. 1967; Transduction in Rhizobium meliloti.. Acta Microbiologica Polonica 16:7–12
    [Google Scholar]
  18. Leong J.M., Nunes-Dueby S.E., Oser A.B., Lesser C.F., Youderian P., Susskind M.M., Landy A. 1986; Structural and regulatory divergence among site-specific recombination genes of lambdoid phage.. Journal of Molecular Biology 189:603–616
    [Google Scholar]
  19. Long S.R. 1989; Rhizobium genetics.. Annual Review of Genetics 23:483–506
    [Google Scholar]
  20. Martin M.O., Long S.R. 1984; Generalized transduction in Rhizobium meliloti.. Journal of Bacteriology 159:125–129
    [Google Scholar]
  21. Sadowski P. 1986; Site-specific recombinases: changing partners and doing the twist.. Journal of Bacteriology 165:341–347
    [Google Scholar]
  22. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Sherwood M.T. 1970; Improved synthetic medium for the growth of Rhizobium.. Journal of Applied Bacteriology 33:708–713
    [Google Scholar]
  24. Sik T., Harwath J., Chatterjee S. 1980; Generalized transduction in Rhizobium meliloti.. Molecular and General Genetics 178:511–516
    [Google Scholar]
  25. Simon R., Preiher U., PÜhler A. 1982; A broad host range mobilization system for in vivo genetic engineering; transposon mutagenesis Gram-negative bacteria.. Biotechnology 1:784–791
    [Google Scholar]
  26. Uchiumi T., Ono Y., Abe M., Higashi S. 1989; Phage induction of lysogenic Rhizobium leguminosarum biovar trifolii in both the free-living and the symbiotic form.. Journal of General Microbiology 135:3133–3141
    [Google Scholar]
  27. Waldman A.S., Fitzmaurice W.P., Scocca J.J. 1986; Integration of the bacteriophage HPlcl genome into the Haemophilus influenzae Rd chromosome in the lysogenic state.. Journal of Bacteriology 165:297–300
    [Google Scholar]
  28. Yamamoto K., Alberts B.M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.. Virology 40:734–744
    [Google Scholar]
  29. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: Nucleotide sequences of the M13mpl8 and pUC19 vectors.. Gene 33:103–119
    [Google Scholar]
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