%0 Journal Article %A Uchiumi, Toshiki %A Higashi, Shiro %A Abe, Mikiko %T A chromosome integrative vector system utilizing DNA fragments of a lysogenic phage of Rhizobium leguminosarum %D 1993 %J Microbiology, %V 139 %N 10 %P 2371-2377 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-10-2371 %I Microbiology Society, %X The attachment site (attP) of phage øU, a lysogenic phage of Rhizohium leguminosarum biovar trifolii, was identified on a 6.0 kb EcoRI fragment of the phage DNA. Plasmid pCI6 was constructed by cloning this EroRI fragment into the EcoRI site of suicide plasmid vector pSUP202. Escherichia coli S17-1 harbouring plasmid pCI6 was mated with wild-type R. leguminosarum biovar trifolii strain 4S and its lysogenic strain 4S(øU) using tetracycline resistance (Tcr) as a selection marker. The Tcr R.leguminosarum biovar trifolii transconjugants appeared at high frequency (10-3-10-4 per recipient cell in both matings). Southern hybridization with the attP fragment and pSUP202 as probes indicated that plasmid pCI6 integrated into the chromosome of all these transconjugants in the same manner as phage øU. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-10-2371