1887

Abstract

SUMMARY: BD413, when grown in batch culture in nutrient broth, produces both extracellular lipase activity and cell-bound esterase activity during and after the transition between exponential growth and the stationary phase. From a library of DNA in , plasmids were isolated that enabled to grow on media with tributyrin as the sole carbon source. Assays with model substrates classified the product of the cloned gene as an esterase. Via deletion analysis, the esterase gene was mapped on a 1.8 kbp chromosomal DNA fragment. This fragment was sequenced and found to contain one open reading frame, termed , which encodes a protein of 40.0 kDa. The amino acid sequence of this protein shows homology to a number of lipolytic enzymes, most notably to esterases. Deletion of only partially abolished cell-bound esterase activity in indicating that BD413 forms at least two esterases. Both esterases show the same temporal regulation of expression. β-Galactosidase activity was measured in strains in which a promoterless gene was inserted into . Induction of expression in these strains also occurred at the end of exponential growth in batch cultures, indicating that production of the esterase is regulated at the genetic level.

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1993-10-01
2021-08-05
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