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Summary: Lactobacillus plantarum strain 4B2, which exhibits a strong autoaggregating phenotype, receives the broad-host-range plasmid pAMβ1 with conjugation efficiencies as high as 10-2 transconjugants per donor using solid matings; broth matings also occur, but at low transfer frequencies. Filter-sterilized spent supernatant of this strain contains a 32 kDa protein that promotes aggregation, and consequently a high frequency of conjugation, in lactic acid bacteria containing α-1,2-glucose-substituted lipoteichoic or teichoic acids. It appears, therefore, that the substituted lipoteichoic or teichoic acids act as receptors for the aggregation-promoting protein.