1887

Abstract

A plant polysaccharide hydrolase cDNA, designated , was isolated from a cDNA library of the rumen fungus . The enzyme encoded by had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that cDNA can be truncated to code for three catalytically active domains. Each domain had the same substrate specificity as the enzyme produced by the untruncated and also possessed cellulose-binding capacity. Substrate competition studies showed that carboxymethylcellulose and xylan appear to compete with methylumbelliferyl cellobioside for the same active site within each domain. Expression of transcript in the rumen fungus was constitutive and was not affected by the presence of cellulose in the culture medium.

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1992-11-01
2024-10-10
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References

  1. Béguin P. 1990; Molecular biology of cellulose degradation. Annual Review of Microbiology 44:219–248
    [Google Scholar]
  2. Berger E., Jones W. A., Jones D. T., Woods D. R. 1989; Cloning and sequencing of an endoglucanase (end-I) gene from Butyrivibrio fibrisolvens Hl7c. Molecular and General Genetics 219:193–198
    [Google Scholar]
  3. El-Gogary S., Leite A., Crivellaro O., Eveleigh D. E., El-Dorry H. 1989; Mechanism by which cellulose triggers cellobiohydrolase I gene expression in Trichoderma reesei. Proceedings of the National Academy of Sciences of the United States of America 866138–0141
    [Google Scholar]
  4. Flint H.J., Mcpherson C. A., Bisset J. 1989; Molecular cloning of genes from Ruminococcus jlavefaciens encoding xylanase and β(1,3-,1,4-)glucanase activities. Applied and Environmental Microbiology 55:1230–1233
    [Google Scholar]
  5. Flint H.J., Mcpherson C. A., Martin J. 1991; Expression of two xylanase genes from the rumen cellulolytic bacterium Ruminococcus jlavefaciens 17 cloned in pUC 13. Journal of General Microbiology 137:123–129
    [Google Scholar]
  6. Foong E., Hamam Oto T., Shoseyov O., Doi R. H. 1991; Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovoram. Journal of General Microbiolog y 137:1729–1736
    [Google Scholar]
  7. Gilbert H.J., Hazlewood G. P., Laurie J. I., Orpin C. G., Xue G. P. 1992; Xylanase A from the rumen anaerobic fungus Neocallimastix patriciarum contains two homologous catalytic domains. Molecular Microbiology (in the Press)
    [Google Scholar]
  8. Gilkes N. R., Langsford M. L., Kilburn D. G., Miller R. C., Warren R. A. J. 1984; Mode of action and substrate specificities of cellulases from cloned bacterial genes. Journal of Biological Chemistry 259:10455–10459
    [Google Scholar]
  9. Gilkes N. R., Warren R. A. J., Miller R. C. Jr, Kilburn D. G. 1988; Precise excision of the cellulose binding domains from two Cellulomonasfimi cellulases by a homologous protease and the effect on catalysis. Journal of Biological Chemistry 263:10401–10407
    [Google Scholar]
  10. Gilkes N. R., Henrissat B., Kilburn D. G., Miller R. C. Jr., Warren R. A. J. 1991; Domains in microbial β-1,4-glycanases : sequence conservation, function, and enzyme families. Microbiological Reviews 55:303–315
    [Google Scholar]
  11. Gomez De Segura B. G., Fevre M. 1991; Cell wall hydrolases from Neocallimastix frontalis: production and regulation. Fourth International Mycological Congress. (Abstracts), p.185
    [Google Scholar]
  12. Goyal A., Ghosh B., Eveleigh D. 1991; Characteristics of fungal cellulases. Bioresource Technology 36:37–50
    [Google Scholar]
  13. Hamamoto T., Shoseyov O., Foong F., Doi R. H. 1990; . A Clostridium cellulovorans gene, engD, codes for both endo-β-1,4-glucanase and cellobiosidase activities. FEMS Microbiology Letters 72:285–288
    [Google Scholar]
  14. Hazlewood G. P., Davidson K., Laurie J. I., Romaniec M. P. M., Gilbert H.J. 1990; Cloning and sequencing of the celA gene encoding endoglucanase A of Butyrivibrio fibrisolvens strain A46. Journal of General Microbiology 136:2089–2097
    [Google Scholar]
  15. Lake B. D., Goodwin H.J. 1976 Lipids. In Chromatographic and Electrophoretic Techniques, vol. I, 4th edn. pp. 345–366 Edited by Smith J., Seakins J. W. T. Bath: Pitman Press;
    [Google Scholar]
  16. Li X., Calza R. E. 1991; Fractionation of cellulases from the ruminal fungus Neocallimastixfrontalis EB188. Applied and Environmental Microbiology 51:3331–3336
    [Google Scholar]
  17. Meinke A., Gilkes N. R., Kilburn O. G., Miller R. C. Jr., Warren R. A. J. 1991; Multiple domains in endoglucanase B (cenB) from Cellulomonas fimi : functions and relatedness to domains in other polypeptides. Journal of Bacteriology 173:7126–7135
    [Google Scholar]
  18. Messner R., Kubicek C. P. 1991; Carbon source control of cellobiohydrolase I and II formation by Trichoderma reesei. Applied and Environmental Microbiology 57:630–635
    [Google Scholar]
  19. Orpin C. G., Munn E. A. 1986; Neocallimastix patriciarum sp. nov., a new member of the Neocallimasticaceae inhabiting the rumen of sheep. Transactions of the British Mycological Society 86:178–180
    [Google Scholar]
  20. Robson L. M., Chambliss G. H. 1989; Cellulases of bacterial origin. Enzyme and Microbial Technology 11:626–644
    [Google Scholar]
  21. Romaniec M. P. M., Davidson K., White B. A., Hazlewood G. P. 1989; Cloning of Ruminococcus albus endo-β-1,4-glucanase and xylanase genes. Letters in Applied Bacteriology 9:101–104
    [Google Scholar]
  22. Saarilajiti H. T., Henrissat B., Palva E. T. 1990; CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora. Gene 90:9–14
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual,, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Saul D. J., Williams L. C., Grayling R. A., Chamley L. W., Love D. R., Bergquist P. L. 1990; CelB, a gene coding for a bifunctional cellulase from the extreme thermophile ’Caldocellum saccharolyticum’. Applied and Environmental Microbiology 56:3117–3124
    [Google Scholar]
  25. Schwarz W. H., Schimming S., Staudenbauer W. L. 1987; High-level expression of Clostridium thermocellum cellulase genes in Escherichia coli. Applied Microbiology and Biotechnology 27:50–56
    [Google Scholar]
  26. Tabor S., Richardson C. 1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 844767–4771
    [Google Scholar]
  27. Taylor K. A., Crosby B., Mcgavin M., Forsberg C. W., Thomas D. Y. 1987; Characteristics of the endoglucanase encoded by a eel gene from Bacteroides succinogenes expressed in Escherichia coli. Applied and Environmental Microbiology 53:41–46
    [Google Scholar]
  28. Teather R. M., Wood P. J. 1982; Use of Congo Red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology 43:777–780
    [Google Scholar]
  29. Tomme P., Van Tilbeurgh H., Pettersson G., Van Damme J., Vandekerckhove J., Knowles J., Teeri T., Claeyssens M. 1988; Studies of the cellulolytic system of Trichoderma reesei QM 9414. European Journal of Biochemistry 170:575–581
    [Google Scholar]
  30. Van Tilbeurgh H., Tomme P., Claeyssens M., Bhikhabhai R., Pettersson G. 1986; Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei. FEBS Letters 204:223–227
    [Google Scholar]
  31. Williams A. G., Orpin C. G. 1987; Polysaccharide-degrading enzymes formed by three species of anaerobic rumen fungi grown on a range of carbohydrate substrates. Canadian Journal of Microbiology 33:418–426
    [Google Scholar]
  32. Xue G. P., Morris R. 1992; Expression of the neuronal surface glycoprotein Thy-I does not follow appearance of its mRNA in developing mouse Purkinje cells. Journal of Neurochemistry 58:430–440
    [Google Scholar]
  33. Xue G. P., Orpin C. G., Gobius K. S., Aylward J. H., Simpson G. D. 1992; Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli. Journal of General Microbiology 138:1413–1420
    [Google Scholar]
  34. Yague E., Beguin P., Aubert J.-P. 1990; Nucleotide sequence and deletion analysis of the cellulase-encoding gene celH of Clostridium thermocellum. Gene 89:61–07
    [Google Scholar]
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