A plant polysaccharide hydrolase cDNA, designated , was isolated from a cDNA library of the rumen fungus . The enzyme encoded by had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that cDNA can be truncated to code for three catalytically active domains. Each domain had the same substrate specificity as the enzyme produced by the untruncated and also possessed cellulose-binding capacity. Substrate competition studies showed that carboxymethylcellulose and xylan appear to compete with methylumbelliferyl cellobioside for the same active site within each domain. Expression of transcript in the rumen fungus was constitutive and was not affected by the presence of cellulose in the culture medium.


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