1887

Abstract

Insertions of the TOL plasmid transposons Tn and Tn into the PAO chromosome were isolated by a temperature selection technique. The locations and orientations of 16 insertions were determined by pulsed field gel electrophoresis and Southern hybridization with genomic and TOL DNA probes. All insertions occurred within a 334 kb region of the chromosome (representing less than 6% of the genome) with nine of the inserts clustered within a 10 kb area. Each transposon was able to insert in either orientation. An internal duplication of the 39 kb excisable region of pWW0 was seen in two independent insertions.

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/content/journal/micro/10.1099/00221287-137-5-1111
1991-05-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-5-1111
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