RT Journal Article SR Electronic(1) A1 Sinclair, M. I. A1 Holloway, B. W.YR 1991 T1 Chromosomal insertion of TOL transposons in Pseudon eruginosa PAO JF Microbiology, VO 137 IS 5 SP 1111 OP 1120 DO https://doi.org/10.1099/00221287-137-5-1111 PB Microbiology Society, SN 1465-2080, AB Insertions of the TOL plasmid transposons Tn4651 and Tn4653 into the Pseudomonas aeruginosa PAO chromosome were isolated by a temperature selection technique. The locations and orientations of 16 insertions were determined by pulsed field gel electrophoresis and Southern hybridization with genomic and TOL DNA probes. All insertions occurred within a 334 kb region of the chromosome (representing less than 6% of the genome) with nine of the inserts clustered within a 10 kb area. Each transposon was able to insert in either orientation. An internal duplication of the 39 kb excisable region of pWW0 was seen in two independent insertions., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-5-1111