An organism able to utilize isonicotinate aerobically as the sole source of carbon, nitrogen and energy was isolated from soil samples and identified as a sp. No growth occurred on any other heteroaromatic substrate tested, except 2-hydroxyisonicotinate, which was shown to be an intermediate in isonicotinate metabolism. Degradation of isonicotinate involved two consecutive hydroxylations leading to 2,6-dihydroxy-isonicotinate (citrazinate). The reactions were catalysed by two different dehydrogenases, which were apparently molybdenum-dependent. Citrazinate was activated by CoA via a synthetase reaction, and the resulting citrazyl-CoA ester was reduced by a NADPH-dependent reductase. In the presence of 1 mM-arsenite, cells accumulated 2-oxoglutarate during isonicotinate metabolism.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error