Summary: nuclease A hybrid genes, encoding proteins OmpA-nuclease, lipo-nuclease and Pin-nuclease, were cloned downstream of the yeast GAL10 inducible promoter. OmpA-nuclease and lipo-nuclease contain the mature staphylococcal nuclease sequence preceded by the OmpA and lipoprotein signal sequences, respectively, whereas Pin-nuclease lacks a defined signal sequence at its amino terminus. We found that: () the nuclease gene products synthesized in yeast are active, but they do not affect cell growth; () OmpA-nuclease and lipo-nuclease are partially processed and constitute approximately 1·0–1·5% of the yeast cell protein; () OmpA and lipoprotein signal sequences function similarly in secretion, allowing 35–40% of the processed nuclease to be translocated into the yeast periplasm; and () Pin-nuclease, which lacks hydrophobic sequences at its amino-terminus, is accumulated at a level tenfold lower than the hybrid proteins that do contain signal sequences. Nevertheless, 50% of the enzyme activity of Pin-nuclease in yeast is localized in the periplasmic space.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error