1887

Abstract

Glycerol is catabolized in by glycerol kinase and a mitochondrial FAD-dependent -glycerol-3-phosphate dehydrogenase. The levels of both enzymes are controlled by carbon catabolite repression and by specific introduction. Biochemical and genetical analyses show that dihydroxyacetone and d-glyceraldehyde are converted into glycerol and then catabolized by the same pathway, -Glyceraldehyde can be reduced by NADP-dependent glycerol dehydrogenase or by alcohol dehydrogenase I, while dihydroxyacetone is only reduced by the first enzyme. Three new glycerol non-utilizing mutants have been found. These three mutations define three hitherto unknown loci, and The mutation in leads to a greatly decreased -glycerol-3-phosphate dehydrogenase activity.

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1991-03-01
2021-10-21
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