1887

Abstract

Binding of purified K99 fimbriae to cryostat sections of pig small intestine was detected. Binding sites were located in the mucus layer, but not in the submucosal connective tissue. High- mucin glycopeptides from pig small intestine were found to bind to K99-fimbriated enterotoxigenic , in contrast to non-fimbriated cells. Sialic acid specificity of K99 fimbriae was demonstrated by the significant reduction in binding upon desialylation of mucin glycopeptides. The binding was saturable and the dissociation constant was estimated to be 6×10 M. Fimbriated bacteria were calculated to possess 2·3×10 binding sites per cell.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-8-1609
1990-08-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/8/mic-136-8-1609.html?itemId=/content/journal/micro/10.1099/00221287-136-8-1609&mimeType=html&fmt=ahah

References

  1. Aronson M., Medalia O., Schori L., Mirelman D., Sharon N., Ofek I. 1979; Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking bacterial adherence with methyl α-d-mannopyranoside. Journal of Infectious Diseases 139:329–332
    [Google Scholar]
  2. Blobel G., Dobberstein B. 1975; Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane bound ribosomes of murine myeloma. Journal of Cell Biology 67:835–851
    [Google Scholar]
  3. Bock K., Breimer M.E., Brignole A., Hansson G.C., Karlsson K.-A., Larson G., Leffler H., Samuelsson B.E., Strömberg N., Svanborg-Edén C., Thurin J. 1985; Specificity of binding of a strain of uropathogenic Escherichia coli to Galαl-4Gal-containing glycosphingolipids. Journal of Biological Chemistry 260:8545–8551
    [Google Scholar]
  4. Brown A.H. 1946; Determination of pentose in the presence of large quantities of glucose. Archives of Biochemistry 11:269–278
    [Google Scholar]
  5. Carlstedt C., Sheehan J.K., Corfield A.P., Gallagher J.T. 1985; Mucous glycoproteins: a gel of a problem. Essays in Biochemistry 20:40–76
    [Google Scholar]
  6. Culling C.F.A., Reid P.E., Dunn W.L., Clay M.G. 1977; Histochemical comparison of the epithelial mucins in the ileum in Crohn’s disease and in normal controls. Journal of Clinical Pathology 30:1063–1067
    [Google Scholar]
  7. Dorner F., Mayer P., Leskova R. 1980; Immunity to Escherichia coli in piglets: the role of colostral antibodies directed against heat labile enterotoxin in experimental neonatal diarrhoea. Zentralblatt für Veterinärmedizin B 27:207–221
    [Google Scholar]
  8. Evans D.G., Evans D.J. JR Clegg S., Pauley J.A. 1979; Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infection and Immunity 25:738–748
    [Google Scholar]
  9. De Graaf F.K., Klemm P., Gaastra W. 1980; Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99. Infection and Immunity 33:877–883
    [Google Scholar]
  10. Guinée P.A.M., Veldkamp J., Jansen W.H. 1977; Improved Minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infection and Immunity 15:676–678
    [Google Scholar]
  11. Hansson G.C., Karlsson K.-A., Larson G., Strömberg N., Thurin J. 1985; Carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors. Analytical Biochemistry 146:158–163
    [Google Scholar]
  12. Hunter W.M. 1978; Radioimmunoassay. In Handbook of Experimental Immunology.14.1–14.40 Weir D. M. Edited by Oxford: Blackwell;
    [Google Scholar]
  13. Jones G.W., Abrams G.D., Freter R. 1976; Adhesive properties of Vibrio cholerae: adhesion to isolated rabbit brush border membranes and hemagglutinating activity. Infection and Immunity 14:232–239
    [Google Scholar]
  14. Jourdain G.W., Dean L., Roseman S. 1971; The sialic acids. XI. A periodic-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. Journal of Biological Chemistry 246:430–435
    [Google Scholar]
  15. Kyogashima M., Ginsburg V., Krivan H.C. 1989; Escherichia coli K99 binds to N-glycolylsialoparagloboside and N-glycolyl-GM3 found in piglet small intestine. Archives of Biochemistry and Biophysics 270:391–397
    [Google Scholar]
  16. Laux D.C., McSweegan E.F., Cohen P.S. 1984; Adhesion of enterotoxigenicEscherichia coli to immobilized intestinal mucosal preparations: a model for adhesion to mucosal surface components. Journal of Microbiological Methods 2:27–39
    [Google Scholar]
  17. Lindahl M., Wadström T. 1986; Binding to erythrocyte membrane glycoproteins and carbohydrate specificity of F41 fimbriae of enterotoxigenic Escherichia coli. FEMS Microbiology Letters 34:297–300
    [Google Scholar]
  18. Lindahl M., Brossmer R., Wadström T. 1987; Carbohydrate receptor specificity of K99 fimbriae of enterotoxigenic Escherichia coli. Glycoconjugate Journal 4:1–9
    [Google Scholar]
  19. Mantle M., Allen A. 1981; Isolation and characterization of the nativt glycoprotein from pig small-intestine mucus. Biochemical Journal 195:267–275
    [Google Scholar]
  20. McQueen S., Hutton D., Allen A., Garner A. 1983; Gastric and duodenal mucus thickness in the rat: effects of prostaglandins and damaging agents. American Journal of Physiology 245:G388–G393
    [Google Scholar]
  21. Morris J.A., Thorns C.J., Sojka W.J. 1980; Evidence for two adhesive antigens on the K99 reference strain Escherichia coli B41. Journal of General Microbiology 118:107–113
    [Google Scholar]
  22. Mouricout M.A., Julien R.A. 1987; Pilus-mediated binding of bovine enterotoxigenic Escherichia coli to small intestinal mucins. Infection and Immunity 55:1216–1223
    [Google Scholar]
  23. Murray P.A., Levine M.J., Tabak L.A., Reddy M.S. 1982; Specificity of salivary-bacterial interactions. II. Evidence for a lectin on Streptococcus sanguis with specificity for a NeuAcα2, 3Galβl, 3GalNAc sequence. Biochemical and Biophysical Research Communications 106:390–396
    [Google Scholar]
  24. Neutra M.R., Forstner J. F. 1987; Gastrointestinal mucus: synthesis, secretion and function. In Physiology of the Gastrointestinal Tract,, 2nd edn.. pp 975–1009 Johnson L. R. Edited by New York: Raven Press;
    [Google Scholar]
  25. Parkkinen J., Finne J., Achtman M., Väisänen V., Korhonen T.K. 1983; Escherichia coli strains binding neuraminyl α2–3 galactosides. Biochemical and Biophysical Research Communications 111:456–461
    [Google Scholar]
  26. Ramphal R., Pyle M. 1983; Evidence for mucins and sialic acid as receptors for Pseudomonas aeruginosa in the lower respiratory tract. Infection and Immunity 41:339–344
    [Google Scholar]
  27. Runnels P.L., Moon H.W., Schneider R.A. 1980; Development of resistance with host age to adhesion of K99+ Escherichia coli to isolated intestinal epithelial cells. Infection and Immunity 28:298–300
    [Google Scholar]
  28. Satterwhite T.K., Evans D.G., DuPont H.L., Evans D.J. JR 1978; Role of Escherichia coli colonization factor antigen in acute diarrhoea. Lancet ii:181–184
    [Google Scholar]
  29. Scatchard G. 1949; The attraction of proteins for small molecules and ions. Annals of the New York Academy of Sciences 51:660–672
    [Google Scholar]
  30. Sheehan J.K., Carlstedt I. 1984; Hydrodynamic properties of human cervical-mucus glycoproteins in 6 m-guanidinium chloride. Biochemical Journal 217:93–101
    [Google Scholar]
  31. Sherman D.M., Acres S.D., Sadowski P.L., Springer J.A., Bray B., Raybould T.J.G., Muscoplat C.C. 1983; Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody. Infection and Immunity 42:653–658
    [Google Scholar]
  32. Smit H., Gaastra W., Kamerling J.P., Vliegenhart J.F.G., De Graaf F.K. 1984; Isolation and structural characterization of the equine erythrocyte receptor for enterotoxigenic Escherichia coli K99 fimbrial adhesin. Infection and Immunity 46:578–584
    [Google Scholar]
  33. Smith H.W., Halls S. 1967; Observation by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. Journal of Pathology and Bacteriology 93:499–529
    [Google Scholar]
  34. Smith H.W., Linggood M.A. 1971; Observation on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. Journal of Medical Microbiology 4:467–485
    [Google Scholar]
  35. Svanborg-Edén C., Freter R., Hagberg L., Hull R., Hull S., Leffler H., Schoolnik G. 1982; Inhibition of experimental ascending urinary tract infection by an epithelial cell-surface receptor analogue. Nature; London: 298560–562
    [Google Scholar]
  36. Wadström T., Smyth C.J., Faris A., Jonsson P., Freer J.H. 1978; Hydrophobic absorptive and hemagglutinating properties of enterotoxigenicEscherichia coli with different colonization factors: K88, K99 and colonization factor antigens and adherence factor. In Proceedings of the 2nd International Symposium on Neonatal Diarrhoea, pp 29–55 Acres S. Edited by Saskatoon: Veterinary Infectious Disease Organization;
    [Google Scholar]
  37. Wagh P.V., Bahl O.P. 1981; Sugar residues on proteins. CRC Critical Reviews in Biochemistry 10:307–377
    [Google Scholar]
  38. Weigel P.H., Schnaar R.L., Kuhlenschmidt M.S., Schmell E., Lee R.T., Lee Y.C., Roseman S. 1979; Adhesion of hepatocytes to immobilized sugars. A threshold phenomenon. Journal of Biological Chemistry 254:10830–10838
    [Google Scholar]
  39. Wu A.M., Pigman W. 1977; Preparation and characterization of armadillo submandibular glycoproteins. Biochemical Journal 161:37–47
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-8-1609
Loading
/content/journal/micro/10.1099/00221287-136-8-1609
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error