1887

Abstract

Differences were detectable among strains of the opportunist fungal pathogen when water-soluble (WS) preparations were analysed by combined SDS-PAGE and Western blotting procedures. A wide range of molecules of apparent molecular masses from approximately 20 to > 100 kDa showed specific binding to antibodies raised in rabbits to wall and cytoplasmic components. The ability to bind antibody was markedly reduced by treatment of these antigens with sodium periodate or with specific proteases or glucanases. Pretreatment of blotted antigens with either concanavalin A (ConA) or wheat germ agglutinin (WGA) did not, however, inhibit subsequent antibody binding. The antigens of subfractions prepared from a single strain of WS material were also susceptible to periodate oxidation and enzymic hydrolysis. Slight cross-reactivity was apparent when crude preparations of cellular or culture filtrate antigens, used in this laboratory to detect antibodies to , Coccidioides immitis and , were probed with hyperimmune rabbit antisera to . Efforts were made to characterize the WS preparations of , used as diagnostic antigens in many laboratories. The electrophoretically separated antigenic moieties were shown to be predominantly glycoproteins. Binding of cytoplasmic antigens to antibodies raised to wall material showed the presence of many common components in both wall and cytosol. Antiserum to wall components revealed most differentiation among strains.

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1990-08-01
2021-05-12
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