1887

Abstract

A DNA fragment containing the locus of , which had been cloned into plasmid pAC3, was subcloned into an M13 phage and sequenced. The sequence contained five open reading frames (ORFs), of which ORF2 was the gene. Unexpectedly, the sequence of the locus was identical to that of [porulation hibition gene; Gaur, N. K., Dubnau, E. & Smith, I. (1986). 168, 860–869]. A homologue () of the locus was cloned into pUC19 and identified by colony hybridization. The DNA was subcloned and sequenced. Two ORFs (ORF1, or L-ORF1; and ORF2, or) were detected, encoding 58 and 111 amino acid residues, respectively. These are almost identical in length to ORF1 (D-ORF1; 57 amino acids) and (111 amino acids) on the fragment of DNA. The overall interspecies differences between the nucleotide sequences of D-ORF1 and L-ORF1, and those of and , were 42% and 11%, respectively, and the differences in the predicted amino acid sequences were 50% and 7%, respectively. The regions 3′ of the ORFs ( and ) in both species resemble rho-independent terminators of transcription. The characteristics of the amino acid sequences are also discussed.

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1990-07-01
2024-12-14
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References

  1. Akamatsu T., Sekiguchi J. 1983; Properties of regeneration mutants of Bacillus subtilis. FEMS Microbiology Letters 20:425–428
    [Google Scholar]
  2. Akamatsu T., Sekiguchi J. 1984; An improved method of protoplast regeneration for Bacillus species and its application to protoplast fusion and transformation. Agricultural and Biological Chemistry 48:651–655
    [Google Scholar]
  3. Akamatsu T., Sekiguchi J. 1987a; Genetic mapping by means of protoplast fusion in Bacillus subtilis. Molecular and General Genetics 208:254–262
    [Google Scholar]
  4. Akamatsu T., Sekiguchi J. 1987b; Genetic mapping and properties of filamentous mutations in Bacillus subtilis. Agricultural and Biological Chemistry 51:2901–2909
    [Google Scholar]
  5. Ayusawa D., Yoneda Y., Yamane K., Maruo B. 1975; Pleiotropic phenomena in autolytic enzyme(s) content, flagellation, and simultaneous hyperproduction of extracellular a-amylase and protease in a Bacillus subtilis mutant. Journal of Bacteriology 124:459–469
    [Google Scholar]
  6. Chatterjee A.N., Wong W., Young F.E., Gilpin R.W. 1976; Isolation and characterization of a mutant of Staphylococcus aureus deficient in autolytic activity. Journal of Bacteriology 125:961–967
    [Google Scholar]
  7. Chou P.Y., Fasman G.D. 1978; Empirical predictions of protein conformation. Annual Review of Biochemistry 47:251–276
    [Google Scholar]
  8. Fein J.E. 1979; Possible involvement of bacterial autolytic enzymes in flagellar morphogenesis. Journal of Bacteriology 137:933–946
    [Google Scholar]
  9. Fein J.E., Rogers H.J. 1976 Autolytic enzyme-deficient mutants of Bacillus subtilis 168, Journal of Bacteriology 127:1427–1442
    [Google Scholar]
  10. Gaur N.K., Dubnau E., Smith I. 1986; Characterization of a cloned Bacillus subtilis gene that inhibits sporulation in multiple copies. Journal of Bacteriology 168:860–869
    [Google Scholar]
  11. Gaur N.K., Cabane K., Smith I. 1988; Structure and expression of the Bacillus subtilis sin operon. Journal of Bacteriology 170:1046–1053
    [Google Scholar]
  12. Gaur N., Mandic-Mulec I., Smith I. 1989; The late growth regulatory gene, sin, encodes a DNA binding protein. In Abstracts of the 5th International Conference on Bacilli, Asilomar abstract no.G-l
    [Google Scholar]
  13. Henner D.J., Ferrari E., Perego M., Hoch J.A. 1988; Upstream activating sequences in Bacillus subtilis. In Genetics and Biotechnology of Bacilli, 2 pp 3–9 Ganesan A. T., Hoch J. A. Edited by New York: Academic Press;
    [Google Scholar]
  14. Kyte J., Doolittle R.F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  15. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Mendelson N.H. 1982; Bacterial growth and division: genes, structures, forces, and clocks. Microbiological Reviews 46:341–375
    [Google Scholar]
  17. Moriyama H., Akamatsu T., Sekiguchi J. 1988; Stable shuttle plasmid, pAC3, among Bacillus subtilis, B. megaterium and Escherichia coli. Nucleic Acids Research 16:8732
    [Google Scholar]
  18. Pooley H., Karamata D. 1984; Genetic analysis of autolysin-deficient and flagellaless mutants of Bacillus subtilis. Journal of Bacteriology 160:1123–1129
    [Google Scholar]
  19. Rose G.D. 1978; Prediction of chain turns in globular proteins on a hydrophobic basis. Nature; London: 212586–590
    [Google Scholar]
  20. Rose G.D., Roy S. 1980; Hydrophobic basis of packing in globular proteins. Proceedings of the National Academy of Sciences of the United States of America 77:4643–4647
    [Google Scholar]
  21. Sekiguchi J., Ezaki B., Kodama K., Akamatsu T. 1988; Molecular cloning of a gene affecting the autolysin level and flagellation in Bacillus subtilis. Journal of General Microbiology 134:1611–1621
    [Google Scholar]
  22. Yang M., Ferrari E., Chen E., Henner D.J. 1986; Identification of the pleiotropic sacQ gene of Bacillus. Journal of Bacteriology 166:113–119
    [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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