Summary: Several inhibitors of ornithine and arginine decarboxylases reduced growth of the fungus cultured on Czapek Dox agar. Of these, the most effective were difluoromethylornithine (DFMO), dehydromonofluoro-methylornithine, difluoromethylarginine and dehydromonofluoromethylarginine. The growth inhibition due to 1 mM-DFMO could be partially reversed with 1 nM-putrescine. Other compounds causing significant reversal of DFMO-mediated growth inhibition included diaminopentane (cadaverine), diaminoheptane, spermidine, 7,7-difluorospermidine, spermine, bis(2-aminoethyl)amine, 2-hydroxy-1,3-diaminopropane, monoacetylputrescine, butenediamine and aminoguanidine. Some compounds, which were relatively innocuous by themselves, increased growth inhibition due to DFMO. Notably effective compounds were methylacetylenicputrescine, aminooxyaminopropane, butynediamine, 2,2-difluoroputrescine, diacetylputrescine, methylglyoxal bis(guanylhydrazone), streptomycin, certain methylated amines, and cyclohexylamine and related compounds. Growth inhibition due to a homologous series of diguanidines [NHC(=NH)NH(CH)NHC(=NH)NH] was also tested. These were especially effective when = 12, and when = 5 or 6. In general, the results suggest that amino-acid-based inhibitors of ornithine decarboxylase have a greater permeability than amine-based inhibitors.


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