Skip to content
1887

Microbiology Society logo Microbiology

Volume 136, Issue 4

Physical map of the aromatic amine and -toluate catabolic plasmid pTDN1 in : location of a unique -cleavage pathway Free

Abstract

A restriction endonuclease map was derived for the aromatic amine and -toluate catabolic plasmid pTDN1 present in UCC22, a derivative of mt-2. The plasmid is 79±1 kbp in size and can be divided into a restriction-site-deficient region of 51±1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in length. A mutant plasmid isolated after growth of the host on benzoate had lost the restriction-profuse region by a straightforward recombinational loss retaining one copy of the direct repeat. Analysis of clones, deletion and Tn insertion mutants strongly suggested that the -cleavage pathway of pTDN1 was situated in the region readily deleted. The catechol 2,3-dioxygenase (C23O) gene of pTDN1 showed no hybridization or restriction homology to previously described C230 genes of TOL plasmids pWW0 and pWW15. In addition, there was little homology between intact pTDN1, pWW0 and pWW15, suggesting the presence of a unique -cleavage pathway. We also demonstrated that pTDN1 did not originate from mt-2 chromosome.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1990
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-4-615
1990-04-01
2025-03-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/4/mic-136-4-615.html?itemId=/content/journal/micro/10.1099/00221287-136-4-615&mimeType=html&fmt=ahah

References

  1. Anson J.G., Mackinnon G. 1984; Novel Pseudomonas plasmid involved in aniline degradation. Applied and Environmental Microbiology 48:868–869
     [Google Scholar]
  2. Assinder S.J., Williams P.A. 1988; Comparison of the meta pathway operons on NAH plasmid pWW60-22 and TOL plasmid pWW53-4 and its evolutionary significance. Journal of General Microbiology 134:2769–2778
     [Google Scholar]
  3. Bachmann B.J. 1987; Derivations and genotypes of some mutant derivatives of Escherichia coli K12. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp 1190–1219 Neidhardt F. C., Ingraham J. L., Brooks Lowe K., Magasanik B., Schaechter M., Umbarger H. E. Edited by Washington, DC: American Society for Microbiology;
     [Google Scholar]
  4. Bagdasarian M., Lurz R., Rückert B., Franklin F.C.H., Bagdasarian M.M., Frey J., Timmis K.N. 1981; Specific-purpose cloning vectors. II. Broad host range, high copy number RSF1010-derived vectors and a host: vector system for gene cloning. Gene 16:237–247
     [Google Scholar]
  5. Barsomian G., Lessie T.G. 1986; Replicon fusions promoted by insertion sequences on Pseudomonas cepacia plasmid pTGL6. Molecular and General Genetics 204:273–280
     [Google Scholar]
  6. Bayley S.A., Duggleby C.J., Worsey M.J., Williams P.A., Hardy K.J., Broda P. 1977; Two modes of loss of the TOL function from Pseudomonas putida mt-2. Molecular and General Genetics 154:203–204
     [Google Scholar]
  7. Birnboim H., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
     [Google Scholar]
  8. Boulnois G.J., Varley J.M., Sharpe G.S., Franklin F.C.H. 1985; Transposon donor plasmids based on colIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria. Molecular and General Genetics 200:65–67
     [Google Scholar]
  9. Cane P.A., Williams P.A. 1986; A restriction map of naphthalene catabolic plasmid pWW60-l and the location of some of its catabolic genes. Journal of General Microbiology 132:2919–2929
     [Google Scholar]
  10. Chatfield L.K., Williams P.A. 1986; Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two non-homologous catechol-2,3-oxygenase genes. Journal of Bacteriology 168:878–885
     [Google Scholar]
  11. Cohen S.N., Chang A.C.Y., Hsu C.L. 1972; Non-chromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
     [Google Scholar]
  12. De La Cruz F., Grinstead J. 1982; Genetic and molecular characterization of Tn27, a multiple resistance transposon from R100.1. Journal of Bacteriology 151:222–228
     [Google Scholar]
  13. Eaton R.W., Ribbons D.W. 1982; Metabolism of dibutylphtha-late and phthalate by Micrococcus sp. strain 12B. Journal of Bacteriology 151:48–57
     [Google Scholar]
  14. Eaton R.W., Timmis K.N. 1986; Spontaneous deletion of a 20 kilobase DNA segment carrying genes specifying isopropyl-benzene metabolism in Pseudomonas putida RE204. Journal of Bacteriology 168:428–430
     [Google Scholar]
  15. Farrell R., Chakrabarty A.M. 1979; Degradative plamids - molecular nature and mode of evolution. In Plasmids of Medical Environmental and Commercial Importance pp 97–109 Timmis K. N., Puhler A. Edited by Amsterdam: Elsevier/North Holland Biomedical Press;
     [Google Scholar]
  16. Franklin F.C.H., Bagdasarian M., Bagdasarian M.M. 1981; Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proceedings of the National Academy of Sciences of the United States of America 78:7458–7462
     [Google Scholar]
  17. Franklin F.C.H., Lehrbach P.R., Lurz R., Rueckert B., Timmis K.N. 1983; Localization and functional analysis of transposon mutations in regulatory genes of the TOL catabolic pathway. Journal of Bacteriology 154:676–685
     [Google Scholar]
  18. Gaffney T.D., Lessie T.G. 1987; Insertion sequence dependent rearrangements of Pseudomonas cepacia plasmid pTGL2. Journal of Bacteriology 169:224–230
     [Google Scholar]
  19. Gasson M.J., Willetts N.S. 1977; Further characterization of the F fertility inhibition systems of “unusual” Fin+ plasmids. Journal of Bacteriology 131:413–420
     [Google Scholar]
  20. Ghosal D., You I.-S., Gunsalus I.C. 1987; Nucleotide sequences and expression of gene nahH of plasmid NAH7 and homology with gene xylE of TOL pWWO. Gene 55:19–28
     [Google Scholar]
  21. Grindley N.D.F. 1985; Transpositional recombination in prokaryotes. Annual Review of Biochemistry 54:863–896
     [Google Scholar]
  22. Grinter N.J. 1983; A broad host range cloning vector transposable to various replicons. Gene 21:133–143
     [Google Scholar]
  23. Guerry P., Le Blanc D.J., Falkow S. 1973; General method for isolation of plasmid deoxyribonucleic acid. Journal of Bacteriology 116:1064–1066
     [Google Scholar]
  24. Harayama S., Lehrbach P.R., Timmis K.N. 1984; Transposon mutagenesis analysis of meta-cleavage pathway operon genes of the TOL plasmid of Pseudomonas putida mt-2. Journal of Bacteriology 160:251–255
     [Google Scholar]
  25. Harayama S., Rekik M., Wasserfallen A., Bairoch A. 1987; Evolutionary relationships between catabolic pathways for aromatics : conservation of gene order and nucleotide sequences of catechol oxidation genes of pWWO and NAH7 plasmids. Molecular and General Genetics 210:241–247
     [Google Scholar]
  26. Holmes D.S., Quigley N. 1981; A rapid boiling method for preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
     [Google Scholar]
  27. Hooper S.W., Dockenforff T.C., Sayler G.S. 1989; Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50. Applied and Environmental Microbiology 55:1286–1288
     [Google Scholar]
  28. Iida S., Meyer J., Arber W. 1983; Prokaryotic IS elements. In Mobile Genetic Elements pp 161–221 Shapiro J. Edited by New York: Academic Press;
     [Google Scholar]
  29. Jorgensen R.A., Rothstein S.J., Reznikoff W.S. 1979; A restriction enzyme cleavage map of TnJ and location of a region encoding neomycin resistance. Molecular and General Genetics 177:65–72
     [Google Scholar]
  30. Keil H., Williams P.A. 1985; A new class of TOL plasmid deletion mutants in Pseudomonas putida MT15 and their reversion by tandem gene amplification. Journal of General Microbiology 131:1023–1033
     [Google Scholar]
  31. Keil H., Lebens M.R., Williams P.A. 1985a; TOL plasmid pWW15 contains two non-homologous independently regulated catechol-2,3-oxygenase genes. Journal of Bacteriology 163:248–255
     [Google Scholar]
  32. Keil H., Keil S., Pickup R.W., Williams P.A. 1985b; Evolutionary conservation of genes coding for meta-pathway enzymes within TOL plasmids pWWO and pWW53. Journal of Bacteriology 164:887–895
     [Google Scholar]
  33. Kleckner N. 1981; Transposable elements in prokaryotes. Annual Review of Genetics 15:341–404
     [Google Scholar]
  34. Kushner S.R. 1978; An improved method for transformation of Escherichia coli with ColEI derived plasmids. In Genetic Engineering pp 17–23 Boyer H. B., Nicoza S. Edited by Amsterdam: Elsevier;
     [Google Scholar]
  35. Lehrbach P.R., McGregor I., Ward J.M., Broda P. 1983; Molecular relationships between Pseudomonas IncP-9 degradative plasmids TOL, NAH, and SAL. Plasmid 10:164–174
     [Google Scholar]
  36. Mcclure N.C., Venables W.A. 1986; Adaptation of Pseudomonas putida mt-2 to growth on aromatic amines. Journal of General Microbiology 132:2209–2218
     [Google Scholar]
  37. Mcclure N.C., Venables W.A. 1987; pTDNl, a catabolic plasmid involved in aromatic amine catabolism in Pseudomonas putida mt-2. Journal of General Microbiology 133:2073–2077
     [Google Scholar]
  38. Meyer R.J., Figurski D., Helinski D. R. 1977; Propertiesof the plasmid RK2 as a cloning vehicle. In DNA Insertion Elements, Plasmids and Episomes pp. 559–566 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  39. Meyer R.J., Shapiro J.A. 1980; Genetic organization of the broad host-range IncP-1 plasmid R751. Journal of Bacteriology 143:1362–1373
     [Google Scholar]
  40. Meulien P., Downing R.G., Broda P. 1981; Excision of the 40 kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats. Molecular and General Genetics 184:97–101
     [Google Scholar]
  41. Murray K., Duggleby C.J., Sala-Trepat J.M., Williams P.A. 1972; The metabolism of benzoate and methyl benzoates via the meta-cleavage pathway by Pseudomonas arvilla mt-2. European Journal of Biochemistry 28:301–310
     [Google Scholar]
  42. Nakai C., Hori K., Kagamiyama H., Nakazawa T., Nozaki M. 1983; Purification, subunit structure, and partial amino acid sequence of metapyrocatechase. Journal of Biological Chemistry 258:2916–2922
     [Google Scholar]
  43. Saint C.P., Venables W.A. 1990; Loss of Tdn catabolic genes by deletion from and curing of plasmid pTDN 1 in Pseudomonas putida: rate and mode of loss are substrate and pH dependent. Journal of General Microbiology 136:627–636
     [Google Scholar]
  44. Shapiro J.A. 1979; Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proceedings of the National Academy of Sciences of the United States of America 76:1933–1937
     [Google Scholar]
  45. Shaw L.E., Williams P.A. 1988; Physical and functional mapping of two cointegrate plasmids derived from RP4 and TOL plasmid pDKl. Journal of General Microbiology 134:2463–2474
     [Google Scholar]
  46. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  47. Stewart G.S.A.B., Lubinski-Mink S., Jackson C.G., Cassel A., Kuhn J. 1986; pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression. Plasmid 15:172–181
     [Google Scholar]
  48. Tsuda M., Iino T. 1987; Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWWO. Molecular and General Genetics 210:270–276
     [Google Scholar]
  49. Wheatcroft R., Williams P.A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas. . Journal of General Microbiology 124:433–437
     [Google Scholar]
  50. Williams P.A., Murray K. 1974; Metabolism of benzoate and the methyl benzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. Journal of Bacteriology 120:416–423
     [Google Scholar]
  51. Worsey M.J., Williams P.A. 1975; Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. Journal of Bacteriology 124:7–13
     [Google Scholar]
  52. Wyndham R.C., Straus N.A. 1988; Chlorobenzoate catabolism and interactions between Alcaligenes and Pseudomonas species from Bloody Run Creek. Archives of Microbiology 150:230–236
     [Google Scholar]
  53. Wyndham R.C., Singh R.K., Straus N.A. 1988; Catabolic instability plasmid gene deletion and recombination in Alcaligenes sp. BR60. Archives of Microbiology 150:237–243
     [Google Scholar]
  54. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
     [Google Scholar]
/content/journal/micro/10.1099/00221287-136-4-615
Loading
Physical map of the aromatic amine and m-toluate catabolic plasmid pTDN1 in Pseudomonas putida: location of a unique meta-cleavage pathway
Microbiology 136, 615 (1990); https://doi.org/10.1099/00221287-136-4-615
/content/journal/micro/10.1099/00221287-136-4-615
/content/journal/micro/10.1099/00221287-136-4-615
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error