1887

Abstract

A protective glycolipid antigen (PAg) was extracted from serovar with chloroform/methanol/water (1:2:0.8, by vol.) and partially purified by silica gel column chromatography. The PAg elicited a protective response in hamsters and in cyclophosphamide-treated mice subsequently challenged with homologous The PAg band was detected as a single smear-like band, corresponding to a protein of 23–30 kDa, by silver-staining in SDS-PAGE. In immunoblots, this band reacted with a monoclonal antibody, A5, which agglutinated serovar and recognized a serovar-specific antigen. Furthermore, the PAg did not migrate on silica gel TLC, but was detected at the origin as a ninhydrin- and naphthol-positive spot. This suggests that PAg is a hydrophilic molecule with a carbohydrate chain that contains amino groups, possibly as amino sugars.

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/content/journal/micro/10.1099/00221287-136-2-327
1990-02-01
2022-01-23
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References

  1. Adler B., Faine S. 1976; Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans serovar pomona . Infection and Immunity 14:703–708
    [Google Scholar]
  2. Adler B., Faine S. 1977; Host immunological mechanisms in the resistance of mice to leptospiral infections. Infection and Immunity 17:67–72
    [Google Scholar]
  3. Auran N. E., Johnson R. C., Ritizi M. D. 1972; Isolation of the outer sheath of Leptospira and its immunogenic properties in hamsters. Infection and Immunity 5:968–975
    [Google Scholar]
  4. Baseman J. B., Cox C. D. 1969; Intermediate energy metabolism of Leptospira . Journal of Bacteriology 97:992–1000
    [Google Scholar]
  5. Bey R. F., Johnson R. C. 1986; Current status of leptospiral vaccines. Progress in Veterinary Microbiology and Immunology 2:175–197
    [Google Scholar]
  6. Bey R. F., Auran N. E., Johnson R. C. 1974; Immunogenicity of whole cell and outer envelope leptospiral vaccines in hamsters. Infection and Immunity 10:1051–1056
    [Google Scholar]
  7. Higashi H., Fukui Y., Ueda S., Kato S., Hirabatashi Y., Matsumoto M., Naiki M. 1984; Sensitive enzyme-immuno-staining and densitometric determination on thin-layer chromatography of N-glycolylneuraminic acid-containing glycosphingolipids, Hanganutziu-Deicher antigen. Journal of Biochemistry 95:1517–1520
    [Google Scholar]
  8. Jost B. H., Adler B., Vinh T., Faine S. 1986; A monoclonal antibody reacting with a determinant on leptospirallipopolysaccharide protects guinea pigs against leptospirosis. Journal of Medical Microbiology 22:269–275
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Masuzawa T., Sekiguchi T., Shimizu T., Iwamoto Y., Morita T., Mifuchi I., Yanagihara Y. 1989a; Monoclonal antibody against Leptospira interrogans serovar canicola . Chemical and Pharmaceutical Bulletin 37:414–417
    [Google Scholar]
  11. Masuzawa T., Nakamura R., Shimizu T., Iwamoto Y., Morita T., Yanagihara Y. 1989b; Immunological characteristics of the glycolipid antigen of Leptospira interrogans serovar lai . Infection and Immunity 57:2502–2506
    [Google Scholar]
  12. Ono E., Takase H., Naiki M., Yanagawa R. 1987; Purification, characterization and serological properties of a glycolipid antigen reactive with a serovar-specific monoclonal antibody against Leptospira interrogans serovar canicola . Journal of General Microbiology 133:1329–1336
    [Google Scholar]
  13. Shimizu T., Matsusaka E., Nagakura N., Takayanagi K., Masuzawa T., Iwamoto Y., Morita T., Mifuchi I., Yanagihara Y. 1987; Chemical properties of lipopolysaccharide- like substance (LLS) extracted from Leptospira interrogans serovar canicola strain Moulton. Microbiology and Immunology 31:717–725
    [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  15. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Analytical Biochemistry 119:115–119
    [Google Scholar]
  16. Yanagihara Y., Masuzawa T., Matsumoto T., Shimizu T., Takayanagi K. 1988; Monoclonal antibody against surface antigen of Leptospira interrogans serovar canicola . Israel Journal of Veterinary Medicine 44:56–58
    [Google Scholar]
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