Summary: The effects of hypersaline treatment (osmotic upshock) on cell water relations were examined in the Gram-positive bacterium using particle size analysis. Application of the Boyle-van't Hoflf relationship (cell volume versus reciprocal of external osmolality) permitted direct determination of turgor pressure, which was approximately 0·75 osmol kg (1·9 MPa) in exponentially growing bacteria in a defined medium. The abolition of turgor pressure immediately after upshock and the subsequent recovery of turgor were investigated. Recovery of turgor was K dependent. Calculation of turgor by an alternative method involving spectrophotometric analysis of shrinkage gave somewhat lower estimates of turgor pressure.


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