Summary: The region of the chromosome was cloned in its natural host in plasmid pHP13. The region contains genes required for glycerol catabolism: coding for glycerol kinase, coding for glycerol-3-phosphate (G3P) dehydrogenase and , proposed to code for a positively acting regulatory protein. The cloned 7 kb fragment carries wild-type alleles of and . It can also complement a strain deleted for the entire region. The wild-type alleles were mapped to different subfragments, establishing the gene order . The nucleotide sequence of and was determined. Immediately upstream of , an additional open reading frame was found, possibly being part of the same operon. Putative transcription terminators were found in the region between and and downstream of . In a coupled in transcription/translation system, two proteins were found, corresponding in size to those predicted from the deduced amino acid sequences of glycerol kinase and G3P dehydrogenase (54 kDa and 63 kDa, respectively).


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