1887

Abstract

Ubiquinones extracted from 24 strains of (including the type strains of serogroups 1–14 and one proposed new serogroup) and from 44 strains of other species (including 28 type strains and strains of five proposed new species) were analysed by reversed-phase thin-layer chromatography. Ubiquinone profiles as determined by this method were reproducible, both qualitatively and semi-quantitatively, and provided information to aid in the identification of species of Results of ubiquinone profiles determined by different laboratories were compared. Some quantitative differences in results between laboratories were observed, which may be due to different analytical procedures. For this reason laboratories should establish their own library of ubiquinone profiles. New information is presented on the ubiquinone profiles of seven species: and proposed new species no. 1347, ‘

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-10-2035
1990-10-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/10/mic-136-10-2035.html?itemId=/content/journal/micro/10.1099/00221287-136-10-2035&mimeType=html&fmt=ahah

References

  1. Abe K., Ishibashi M., Ohmae M., Kawabe K., Katsui G. 1978; Determination of ubiquinone in serum and liver by high speed liquid chromatography. Journal of Nutritional Science and Vitaminology 24:555–567
    [Google Scholar]
  2. Bornstein N., Marmet D., Surgot M., Nowicki M., Meugnier H., Fleurette J., Ageron E., Grimont F., Grimont P.A.D., Thacker W.L., Benson R.F., Brenner D.J. 1989; Legionella gratiana sp. nov. isolated from French spa water. Research in Microbiology 140:541–552
    [Google Scholar]
  3. Brenner D.J., Steigerwalt A.G., Epple P., Bibb W.F., Mckinney R.M., Starnes R.W., Colville J.M., Selander R.K., Edelstein P.H., Moss C.W. 1988; Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp.pneumophila subsp. nov., L. pneumophila subsp.raseri subsp. nov., and L. pneumophila subsp.pascullei subsp. nov. Journal of Clinical Microbiology 26:1695–1703
    [Google Scholar]
  4. Collins M.D., Gilbart J. 1983; New members of the coenzyme Q series from the Legionellaceae. FEMS Microbiology Letters 16:251–255
    [Google Scholar]
  5. Collins M.D., Jones D. 1981; A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin layer chromatography and high performance chromatography. Journal of Applied Bacteriology 51:129–134
    [Google Scholar]
  6. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. 1977; Distribution of menaquinones in actinomycetes and coryne- bacteria. Journal of General Microbiology 100:221–230
    [Google Scholar]
  7. Edelstein P.H. 1981; Improved semi-selective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. Journal of Clinical Microbiology 14:298–303
    [Google Scholar]
  8. Gilbart J., Collins M.D. 1985; High performance liquid chromatographic analysis of ubiquinones from new Legionella species. FEMS Microbiology Letters 26:77–82
    [Google Scholar]
  9. Harrison T.G., Saunders N.A., Doshi N., Wait R. 1988; Serological diversity within the species Legionella spiritensis. Journal of Applied Bacteriology 65:425–431
    [Google Scholar]
  10. Karr D.E., Bibb W.F., Moss C.W. 1982; Isoprenoid quinonesof the genus Legionella. Journal of Clinical Microbiology 14:1044–1048
    [Google Scholar]
  11. Krivankova L., Dadak V. 1980; Semimicro extraction of ubiquinones and menaquinones from bacteria. Methods in Enzymology 67:111–114
    [Google Scholar]
  12. Lambert M.A., Moss W.C. 1989; Cellular fatty acid compositions and isoprenoid quinone contents of 23 Legionella species. Journal of Clinical Microbiology 27:465–473
    [Google Scholar]
  13. Moss C.W., Bibb W.F., Karr D.E., Guerrant G.O., Lambert M.A. 1983; Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. Journal of Clinical Microbiology 18:917–919
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-10-2035
Loading
/content/journal/micro/10.1099/00221287-136-10-2035
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error