1887

Abstract

Gold-labelled rabbit antiserum was used to demonstrate that a cuticle-degrading protease (Pr1) is produced by during penetration of host () procuticle. The protease was secreted by infection structures (appressoria) on the cuticle surface and by the penetrant hyphae within the cuticle. Penetration of the procuticle was by a combination of enzymic degradation and mechanical pressure. Initially Pr1 was confined to the immediate vicinity of the fungal structures; however the enzyme diffused throughout the cuticle during later stages of pathogenesis. When hyphae were labelled during growth in culture under conditions conducive to rapid synthesis of Pr1, gold particles distributed over the fungal cell wall, indicating binding of Pr1 to hyphae.

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/content/journal/micro/10.1099/00221287-135-8-2233
1989-08-01
2022-01-20
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