SUMMARY: In the mutant BEN210 of several β-tubulins are detectable. β-tubulin is unique to the myxamoeba, β-tubulin is unique to the plasmodium, and the mutant β-210 tubulin encoded by the allele is present in both cell types. In order to analyse the subcellular distribution of the β-210 polypeptide, we prepared cytoskeletons from myxamoebae and mitotic spindles from plasmodia, and examined the tubulin polypeptide composition of these microtubular organelles by two-dimensional gel electrophoresis and immunoblotting. The results show that the β-210 tubulin is present in microtubules of both the cytoskeleton and the intranuclear mitotic spindle. Thus a single β-tubulin gene product can participate in multiple microtubular organelles in distinct cellular compartments.


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