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In the mutant BEN210 of Physarum polycephalum several β-tubulins are detectable. β1-tubulin is unique to the myxamoeba, β2-tubulin is unique to the plasmodium, and the mutant β1-210 tubulin encoded by the benD210 allele is present in both cell types. In order to analyse the subcellular distribution of the β1-210 polypeptide, we prepared cytoskeletons from myxamoebae and mitotic spindles from plasmodia, and examined the tubulin polypeptide composition of these microtubular organelles by two-dimensional gel electrophoresis and immunoblotting. The results show that the β1-210 tubulin is present in microtubules of both the cytoskeleton and the intranuclear mitotic spindle. Thus a single β-tubulin gene product can participate in multiple microtubular organelles in distinct cellular compartments.
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