1887

Abstract

In the mutant BEN210 of several -tubulins are detectable. 1-tubulin is unique to the myxamoeba, 2-tubulin is unique to the plasmodium, and the mutant 1-210 tubulin encoded by the allele is present in both cell types. In order to analyse the subcellular distribution of the 1-210 polypeptide, we prepared cytoskeletons from myxamoebae and mitotic spindles from plasmodia, and examined the tubulin polypeptide composition of these microtubular organelles by two-dimensional gel electrophoresis and immunoblotting. The results show that the 1-210 tubulin is present in microtubules of both the cytoskeleton and the intranuclear mitotic spindle. Thus a single -tubulin gene product can participate in multiple microtubular organelles in distinct cellular compartments.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-3-623
1989-03-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/3/mic-135-3-623.html?itemId=/content/journal/micro/10.1099/00221287-135-3-623&mimeType=html&fmt=ahah

References

  1. Adachi Y., Toda T., Niwa W., Yanagida M. 1986; Differential expression of essential and nonessential α-tubulin genes in Schizosaccharomyces pombe . Molecular and Cellular Biology 6:2168–2178
    [Google Scholar]
  2. Birkett C.R., Foster K.E., Johnson L., Gull K. 1985; Use of monoclonal antibodies to analyze the expression of a multigene family. FEBS Letters 187:211–218
    [Google Scholar]
  3. Burland T.G., Schedl T., Gull K., Dove W.F. 1984; Genetic analysis of resistance to benzimid-azoles in Physarum: differential expression of β-tubulin genes. Genetics 108:123–141
    [Google Scholar]
  4. Burland T.G., Paul E.C.A., Oetliker M., Dove W.F. 1988; A gene encoding the major /f-tubulin of the mitotic spindle in Physarum polycepha-lum plasmodia. Molecular and Cellular Biology 8:1275–1281
    [Google Scholar]
  5. Clayton L.C., Pogson C.I., Gull K. 1982; Ultrastructural and biochemical characterization of Physarum polycephalum myxamoebae. Protoplasma 118:181–191
    [Google Scholar]
  6. Cleveland D.W., Sullivan K. 1985; Molecular biology of tubulin. Annual Review of Biochemistry 54:331–365
    [Google Scholar]
  7. Cooke D.J., Dee J. 1975; Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum . Genetical Research 24:175–187
    [Google Scholar]
  8. Foster K.E., Burland T.G., Gull K. 1987; A mutant β-tubulin confers resistance to the action of benzimidazole-carbamate inhibitors both in vivo and in vitro . European Journal of Cell Biology 163:449–455
    [Google Scholar]
  9. Fulton C., Simpson P.A. 1976; Selective synthesis and utilization of flagellar tubulin. The multitubulin hypothesis. In Cell Motility pp. Goldman R., Pollard T., Rosenbaum J. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Gard D.L., Kirschner M.W. 1985; A polymer-dependent increase in phosphorylation of ^-tubulin accompanies differentiation of a mouse neuroblas-toma cell line. Journal of Cell Biology 100:764–774
    [Google Scholar]
  11. Hancock K., Tsang V.C.W. 1983; India ink staining of proteins on nitrocellulose paper. Analytical Biochemistry 133:157–162
    [Google Scholar]
  12. Kemphues K.J., Kaufman T.C., Raff R.A., Raff E.C. 1982; The testis-specific beta tubulin subunit in Drosophila melanogaster has multiple functions in spermatogenesis. Cell 31:655–670
    [Google Scholar]
  13. Lewis S.A., Gu W., Cowan N.J. 1987; Free intermingling of mammalian β-tubulin isotypes among functionally distinct microtubules. Cell 49:539–548
    [Google Scholar]
  14. Lopata M.A., Cleveland D.W. 1988; In vivo microtubules are copolymers of available β-tubulin isotypes: localization of each of six vertebrate β-tubulin isotypes using polyclonal antibodies elicited by synthetic peptide antigens. Journal of Cell Biology 105:1707–1720
    [Google Scholar]
  15. May G.S., Gambino J., Weatherbee J.A., Morris N.R. 1985; Identification and functional analysis of beta-tubulin genes by site specific integrative transformation in Aspergillus nidulans . Journal of Cell Biology 101:712–719
    [Google Scholar]
  16. Mccullough C.H.R., Dee J., Foxon J.L. 1978; Genetic factors determining growth of Physarum polycephalum amoebae in axenic medium. Journal of General Microbiology 106:297–306
    [Google Scholar]
  17. Parysek L.M., Wolosewick J.J., Olmsted J.B. 1984; MAP4: a microtubule-associated protein specific for a subset of tissue microtubules. Journal of Cell Biology 99:2287–2296
    [Google Scholar]
  18. Paul E.C.A., Roobol A., Foster K.E., Gull K. 1987; Patterns of tubulin isotype synthesis and usage during mitotic spindle morphogenesis in Physarum . Cell Motility and the Cytoskeleton 7:272–281
    [Google Scholar]
  19. Piperno G., Fuller M.T. 1985; Monoclonal antibodies specific for acetylated form of α-tubulin recognize the antigen in cilia and flagella from a variety of organisms. Journal of Cell Biology 101:2085–2094
    [Google Scholar]
  20. Raybin D., Flavin M. 1977; Modification of tubulin by tyrosylation in cells and extracts and its effects on assembly in vitro . Journal of Cell Biology 73:492–504
    [Google Scholar]
  21. Roobol A., Pogson C.I., Gull K. 1980; In vitro assembly of microtubule proteins from myxamoebae of Physarum polycephalum . Experimental Cell Research 130:203–215
    [Google Scholar]
  22. Roobol A., Paul E.C.A., Birkett C.R., Foster K.E., Gull K., Burland T.G., Dove W.F., Green L., Johnson L., Schedl T. 1984a; Cell types, microtubular organelles, and the tubulin gene families of Physarum . In Molecular Biology of the Cytoskeleton pp. 223–234 Borisy G. G., Cleveland D. W., Murphy D. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Roobol A., Paul E.C.A., Gull K. 1984; b)The isolation of nuclei containing mitotic spindles from the slime mould Physarum polycephalum . European Journal of Cell Biology 33:24–29
    [Google Scholar]
  24. Schatz P.J., Solomon F., Botstein D. 1986; Genetically essential and nonessential α-tubulin genes specify functionally interchangeable proteins. Molecular and Cellular Biology 6:3722–3733
    [Google Scholar]
  25. Schedl T., Owens J., Dove W.F., Burland T.G. 1984; Genetics of the tubulin gene families of Physarum . Genetics 108:143–164
    [Google Scholar]
  26. Singhofer-Wowra M.L., Clayton L.C., Dawson P., Gull K., Little M. 1986; Amino acid sequence data of β-tubulin from Physarum polycephalum myxamoebae. European Journal of Cell Biology 161:669–679
    [Google Scholar]
  27. Towbin M., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  28. Weatherbee J.A., May G.S., Gambino J., Morris N.R. 1985; Involvement of a particular species of beta-tubulin (Beta3) in conidial development in Aspergillus nidulans . Journal of Cell Biology 101:706–711
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-3-623
Loading
/content/journal/micro/10.1099/00221287-135-3-623
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error