%0 Journal Article %A Paul, Eileen C. A. %A Burland, Timothy G. %A Gull, Keith %T Location of a Single β-Tubulin Gene Product in Both Cytoskeletal and Mitotic-spindle Microtubules in Physarum polycephalum %D 1989 %J Microbiology, %V 135 %N 3 %P 623-628 %@ 1465-2080 %R https://doi.org/10.1099/00221287-135-3-623 %I Microbiology Society, %X In the mutant BEN210 of Physarum polycephalum several β-tubulins are detectable. β1-tubulin is unique to the myxamoeba, β2-tubulin is unique to the plasmodium, and the mutant β1-210 tubulin encoded by the benD210 allele is present in both cell types. In order to analyse the subcellular distribution of the β1-210 polypeptide, we prepared cytoskeletons from myxamoebae and mitotic spindles from plasmodia, and examined the tubulin polypeptide composition of these microtubular organelles by two-dimensional gel electrophoresis and immunoblotting. The results show that the β1-210 tubulin is present in microtubules of both the cytoskeleton and the intranuclear mitotic spindle. Thus a single β-tubulin gene product can participate in multiple microtubular organelles in distinct cellular compartments. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-3-623