1887

Abstract

The effects of culture conditions (aerobic or anaerobic) and glucose in the medium on the production of spirosomes in B were studied by SDS-PAGE and electron microscopy. The of the spirosome of B was estimated to be 97000. Electron microscopy revealed that the amount of spirosomes derived from anaerobic cultures was about eightfold larger than that from aerobic cultures. In SDS-PAGE, the bands of spirosome protein derived from anaerobic cultures were more intense than those derived from aerobic cultures, either in peptone water or in Davis-Mingioli’s minimal medium. With increased glucose concentration under aerobic conditions, the intensity of the band of spirosome protein was similar to that observed under anaerobic conditions in basal media. These results suggest that spirosome production by B is related to its anaerobic glycolysis activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-3-525
1989-03-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/3/mic-135-3-525.html?itemId=/content/journal/micro/10.1099/00221287-135-3-525&mimeType=html&fmt=ahah

References

  1. Hempfling W.P. 1970; Repression of oxidative phosphorylation in Escherichia coli B by growth in glucose and other carbohydrates. Biochemical and Biophysical Research Communications 41:9–15
    [Google Scholar]
  2. Holt S.C., Canale-Parola E. 1968; Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete. Journal of Bacteriology 96:822–835
    [Google Scholar]
  3. Kawata T. 1984; Spirosomes-spiral inclusion bodies in bacteria. Denshikenbikyo 19:3–9 (in Japanese)
    [Google Scholar]
  4. Kawata T., Masuda K., Yoshino K. 1975; Presence of fine spirals (spirosomes) in Lactobacillus fermenti and Lactobacillus casei . Japanese Journal of Microbiology 19:225–227
    [Google Scholar]
  5. Kawata T., Masuda K., Ueki Y. 1976; Isolation and ultrastructural characterization of fine spirals (spirosome) from Lactobacillus brevis . Journal of Electron Microscopy 25:283–288
    [Google Scholar]
  6. Kawata T., Masuda K., Ueki Y. 1979; Presence of spirosomes in Escherichia coli . Microbiology and Immunology 23:555–558
    [Google Scholar]
  7. Kawata T., Masuda K., Nomura S. 1982; Superprecipitation-like phenomenon and destruction induced by adenosine 5´-triphosphate in spirosomes isolated from Lactobacillus brevis . Microbiology and Immunology 26:979–983
    [Google Scholar]
  8. Kawata T., Nomura S., Hurukawa T., Masuda K. 1986; Isolation and characteristics of spirosomes in species of Acholeplasma . Japanese Journal of Bacteriology 41:557 (in Japanese)
    [Google Scholar]
  9. Kessel M., Peleg I., Muhlrad A., Kahane I. 1981; Cytoplasmic helical structure associated with Acholeplasma laidlawii . Journal of Bacteriology 147:653–659
    [Google Scholar]
  10. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Matayoshi S., Oda H. 1985; Detection of fine spiral structures (spirosomes) by weak sonication in some bacterial strains. Microbiology and Immunology 29:13–20
    [Google Scholar]
  12. Nieves B.M., Gil F., Castillo F.J. 1981; Growth inhibition activity and bacteriophage and bacteriocin-like particles associated with different species of Clostridium . Canadian Journal of Microbiology 27:216–225
    [Google Scholar]
  13. Spear P.G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. Journal of Virology 9:143–159
    [Google Scholar]
  14. Ueda M., Takagi A. 1972; Rhapidosomes in Clostridium botulinum . Journal of General and Applied Microbiology 18:81–98
    [Google Scholar]
  15. Ueki Y., Masuda K., Kawata T. 1982; Purification and characterization of spirosomes in Lactobacillus brevis . Microbiology and Immunology 26:199–211
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-3-525
Loading
/content/journal/micro/10.1099/00221287-135-3-525
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error