1887

Abstract

The phosphate regulation and subcellular location of the hydrolytic enzyme alkaline phosphatase were investigated in the Gram-negative bacterium . The biosynthesis of alkaline phosphatase was not derepressed at a low phosphate concentration as is generally observed in other micro-organisms, nor was it repressed by high phosphate concentrations in the medium. The enzyme level was rather constant during the growth phases in batch culture, at a value at least 8.4-fold lower than that observed in . The alkaline phosphatase of was found associated with the membrane fraction after cell disruption, osmotic shock treatment or spheroplast formation. This is a rather unusual location, since most of the alkaline phosphatases from Gram-negative bacteria have been shown to be periplasmic enzymes. Activity staining on polyacrylamide gels after two-dimensional electrophoresis revealed two isoforms of alkaline phosphatase, each of approximate molecular mass 56 kDa. These two forms belong to a group including the more basic envelope proteins of . Our results indicate that the enzyme from differs markedly from typical alkaline phosphatases of other Gram-negative bacteria.

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1989-02-01
2021-10-24
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References

  1. Ames B., Dubin J.D. 1960; Role of polyamines in the neutralization of bacteriophage DNA. Journal of Biological Chemistry 235:769–775
    [Google Scholar]
  2. Azoulay T. 1986 Effets de la chaleur et de l’éthanol sur Zymomonas mobilis Thèse Université, d’Aix-Marseille II, France:
    [Google Scholar]
  3. Baratti J., Bu’Lock J.D. 1986; Zymomonas mobilis: a bacterium for ethanol production. Biotechnology Advances 4:95–115
    [Google Scholar]
  4. Beckwith J. 1987; Protein secretion and use of alkaline phosphatase. Secretion of phosphate-related proteins. In Phosphate Metabolism and Cellular Regulation in Microorganisms pp 71–72 Torriani-Gorini A., Rothman F. G., Silver S., Wright A., Yagil E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Bhatti A.R., De Voe I.W., Ingram J.M. 1976; The release and characterization of some periplasm- located enzymes of Pseudomonas aeruginosa . Canadian Journal of Microbiology 22:1425–1429
    [Google Scholar]
  6. Brestic-Goachet N. 1988 Amélioration génétiquede Zymomonas mobilis: mutagénèse et transfert de gènes d’hydrolases Thèse Université d’Aix-Marseille I, France.:
    [Google Scholar]
  7. Bringer S., Hartner T., Poralla K., Sahm H. 1985; Influence of ethanol on the hopanoid content and the fatty acid pattern in batch and continuous cultures of Zymomonas mobilis . Archives of Microbiology 140:312–316
    [Google Scholar]
  8. Cheng K.J., Costerton J.W. 1973; Localization of alkaline phosphatase in three Gram-negative rumen bacteria. Journal, of Bacteriology 116:424–440
    [Google Scholar]
  9. Cheng K.J., Ingram J.M., Costerton J.W. 1970a; Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and pH. Journal of Bacteriology 104:748–753
    [Google Scholar]
  10. Cheng K.J., Ingram J.M., Costerton J.W. 1970b; Alkaline phosphatase localization and spheroplast formation of Pseudomonas aeruginosa . Canadian Journal of Microbiology 16:1319–1324
    [Google Scholar]
  11. Chopra I., Shales S.J.S. 1980; Comparison of polypeptide composition of E.coli outer membranes prepared by two methods. Journal of Bacteriology 144:425–427
    [Google Scholar]
  12. Doelle H.W., Preusser H.J., Rostek M. 1982; Electron microscopic investigations of Z.mobilis cells grown in low and high glucose concentrations. European Journal of Applied Microbiology and Biotechnology 16:136–141
    [Google Scholar]
  13. Enequist H.C., Hirst T.R., Harayama S., Hardy S.J.S., Randall L.L. 1981; Energy is required for maturation of exported proteins in Escherichia coli . European Journal of Biochemistry 116:227–233
    [Google Scholar]
  14. Filip C., Fletcher G., Wulff J.L., Earhart C.F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli in exponential phase. Journal of Bacteriology 115:717–722
    [Google Scholar]
  15. Garen A., Levinthal C. 1960; A fine structure genetic and chemical study of the enzyme alkaline phosphatase. Biochimica et biophysica acta 38:470–483
    [Google Scholar]
  16. Ingram L.O., Buttke T.M. 1984; Effect of alcohols on microorganisms. Advances in Microbial Physiology 25:254–290
    [Google Scholar]
  17. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  18. Michel G.P.F., Azoulay T., Starka J. 1985; Ethanol effect on the membrane protein pattern of Zymomonas mobilis . Annales de l’Institut Pasteur/Microbiologie 136A:173–179
    [Google Scholar]
  19. Montenecourt B.S. 1985; Zymomonas, a unique genus of bacteria. In Biology of Industrial Microorganisms pp 261–269 Demain A. L., Salomon N. A. Edited by Menlo Park: Benjamin & Cummings;
    [Google Scholar]
  20. Neu H.C., Heppel L.A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  21. Nossal N.G., Heppel L.A. 1966; The release of enzymes by osmotic shock from Escherichia coli in exponential phase. Journal of Biological Chemistry 241:3055–3062
    [Google Scholar]
  22. O’Farrell P.H. 1975; High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  23. Osborn M.J., Gander J.E., Parisi E., Carson J. 1972; Mechanism and assembly of the outer membrane of S.typhimurium. Isolation and characterization of cytoplasmic and outer membrane. Journal of Biological Chemistry 247:3962–3972
    [Google Scholar]
  24. Poirier T.P., Holt S.C. 1983; Acid and alkaline phosphatases of Capnocytophaga species. I. Production and cytological localization of the enzymes. Canadian Journal of Microbiology 29:1350–1360
    [Google Scholar]
  25. Randall L.L., Hardy S.J.S., Thom J.R. 1987; Export of proteins: a biochemical view. Annual Review of Microbiology 41:507–541
    [Google Scholar]
  26. Schaecterle G.R., Pollach R.L. 1975; A simplified method for the quantitative assay of protein in biological material. Analytical Biochemistry 51:654–655
    [Google Scholar]
  27. Swings J., De Ley J. 1977; The biology of Zymomonas . Bacteriological Reviews 41:1–46
    [Google Scholar]
  28. Tomabene T.G., Holzer G., Bittner A.S., Grohman K. 1982; Characterization of the total extractable lipids of Z. mobilis . Canadian Journal of Microbiology 28:1107–1118
    [Google Scholar]
  29. Torriani A. 1960; Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli . Biochimica et biophysica acta 38:460–479
    [Google Scholar]
  30. Torriani A. 1966; Alkaiine phosphatase from Escherichia coli . In Procedures in Nucleic Acid Research pp 224–235 Cantoni G. L., Davis D. R. Edited by Harper and Row
    [Google Scholar]
  31. Von Tigerstrom R.G. 1984; Production of two phosphatases by Lysobacter enzymogenes and purification and characterization of the extracellular enzyme. Applied and Environmental Microbiology 47:693–698
    [Google Scholar]
  32. Von Tigerstrom R.G., Stelmaschuk S. 1985; Localization of the cell-associated phosphatase in Lysobacter enzymogenes . Journal of General Microbiology 131:1611–1618
    [Google Scholar]
  33. Welsch M. 1958; Formation de protoplastes d’Escherichia coli sous l’influence de la glycine et d’autres acides amines. Pathologie und Bakteriologie 21:741–768
    [Google Scholar]
  34. Willis R.C., Morris R.G., Cirakoglu C., Schellenberg G.D., Gerber N.H., Furlong C.E. 1974; Preparation of the periplasmic binding proteins of Salmonella typhimurium and Escherichia coli . Archives of Biochemistry and Biophysics 161:64–75
    [Google Scholar]
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