SUMMARY: Isogenic 2 and WHI+ strains of were grown in a 2-litre bioreactor as batch cultures on a medium containing yeast extract and peptone with either glucose or ethanol as carbon and energy source. The concentration of dissolved oxygen within the medium was varied over the range of 0 to 100% saturation. Expression of the 2 phenotype only occurred above 40% oxygen saturation with either glucose or ethanol as carbon and energy source. Under these conditions the 2 cells could be distinguished from WHI2+ cells in that they were phase dark, highly budded and very small during the stationary growth phase, and reached final cell densities four to six times higher than WHI2+ cells. The results clearly show that the WHI2 gene of plays an important role in cell proliferation and that the availability of oxygen, or some product of oxidative metabolism, is involved in regulating the phenotypic expression of mutations within this gene.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error