1887

Abstract

The DNA of an isolate of , an agent of relapsing fever is present as seven major species ranging in size from 10 kb to greater than 150 kb. Additionally, this isolate contains low copy number species, both smaller and larger than these seven major elements. No one of these individual DNA species obviously corresponds to the bacterial chromosome, unlike the situation in , another relapsing fever . Thus it appears that has a unique segmented arrangement of its genetic material. Cloned DNA fragments containing coding sequences specific for variant surface antigens of hybridize to a closely migrating, high copy number subset of these genetic elements.

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/content/journal/micro/10.1099/00221287-134-7-1785
1988-07-01
2024-10-10
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References

  1. Barstad P. A., Coligan J. E., Raum M. G., Barbour A. G. 1985; Variable major proteins of Borrelia hermsii: epitope mapping and partial sequence analysis of CNBr peptides. Journal of Experimental Medicine 161:1302–1314
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; Rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Borst P. 1986; Genee rearrangements controlling gene expression. Biochemistry International 12:567–591
    [Google Scholar]
  4. Burgdorfer W. 1976; The epidemiology of relapsing fevers. In The Biology of Parasitic Spirochaetes pp. 191–200 Johnson Russell C. Edited by London: Academic Press;
    [Google Scholar]
  5. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  6. Felsenfeld O. 1971; Human borreliosis (relapsing fever). In Borrelia: Strains, Vectors, Human and Animal Borreliosis pp. 98–112 St Louis: Warren H. Green;
    [Google Scholar]
  7. Hanahan D. 1985; Techniques for transformation of E. coli. In DNA Cloning 1 531 pp. 109–135 Glover D. M. Edited by Oxford: IRL Press;
    [Google Scholar]
  8. Hoeijimakers J. H. J., Frasch A. C. C., Bernards A., Borst P., Cross G. A. M. 1980; Novel expression-linked copies of the genes for variant surface antigens in trypanosomes. Nature; London: 28478–80
    [Google Scholar]
  9. Hyde F. W., Johnson R. C. 1984; Genetic relationship of Lyme disease spirochetes to Borrelia, Treponema, and Leptospira spp. Journal of Clinical Microbiology 20:151–154
    [Google Scholar]
  10. Ish-Horowicz D., Burke J. R. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  11. Kinashi H., Shimaji M., Sakai A. 1987; Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes. Nature; London: 328454–456
    [Google Scholar]
  12. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual pp. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Meier J. T., Simon M. I., Barbour A. G. 1985; Antigenic variation is associated with DNA rearrangements in a relapsing fever Borrelia. Cell 41:403–409
    [Google Scholar]
  14. Norgard M. V., Miller J. N. 1981; Plasmid DNA in Treponema pallidum (Nichols): potential for antibiotic resistance by syphilis bacteria. Science 213:553–555
    [Google Scholar]
  15. Plasterk R. H., Simon M. I., Barbour A. G. 1985; Transposition of structural genes to an expression sequence on a linear plasmid causes antigenic variation in the bacterium Borrelia hermsii. Nature; London: 318257–263
    [Google Scholar]
  16. Porter C. D., Porter A. G. F., Archard L. C. 1988; BBC microcomputer controlled field inversion gel electrophoresis. Computer Applications in the Biosciences 4:271–273
    [Google Scholar]
  17. Schwartz D. C., Cantor C. R. 1984; Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Stoenner H. G., Dodd T., Larsen C. 1982; Antigenic variation of Borrelia hermsii. Journal of Experimental Medicine 156:1297–1311
    [Google Scholar]
  20. Van der ploeg L. H. T., Cornelissen A.W.C.A., Barry J. D., Borst P. 1984; Chromosomes of kinetoplastida. EMBO Journal 3:3109–3115
    [Google Scholar]
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