1887

Abstract

Summary: The recently described extremely thermophilic sulphate-reducing archaebacterium “VC-16” has been shown to contain adenylylsulphate reductase (EC 1.8.99.2). The enzyme was purified 26-fold by ammonium sulphate fractionation, DEAE-cellulose chromatography, hydrophobic chromatography and preparative isoelectric focusing. The enzyme preparation gave a single band on analysis by SDS-PAGE. The was 160000 and the enzyme contained 1 mol FAD, 8 mols non-haem iron and 6 mols labile sulphide per mol enzyme. Ferricyanide was used as electron acceptor. The optimal reaction temperature was 857°C under the assay conditions used and the pH optimum of the enzyme reaction was 8.0. The values for AMP and ferricyanide were 1 mM and 0.4 mM, respectively. The pI of the archaebacterial adenylylsulphate reductase was 4.8.

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/content/journal/micro/10.1099/00221287-134-6-1419
1988-06-01
2019-10-15
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