RT Journal Article SR Electronic(1) A1 Speich, Norbert A1 Trüper, Hans G.YR 1988 T1 Adenylylsulphate Reductase in a Dissimilatory Sulphate-reducing Archaebacterium JF Microbiology, VO 134 IS 6 SP 1419 OP 1425 DO https://doi.org/10.1099/00221287-134-6-1419 PB Microbiology Society, SN 1465-2080, AB The recently described extremely thermophilic sulphate-reducing archaebacterium ‘VC-16’ has been shown to contain adenylylsulphate reductase (EC 1.8.99.2). The enzyme was purified 26-fold by ammonium sulphate fractionation, DEAE-cellulose chromatography, hydrophobic chromatography and preparative isoelectric focusing. The enzyme preparation gave a single band on analysis by SDS-PAGE. The M r was 160000 and the enzyme contained 1 mol FAD, 8 mols non-haem iron and 6 mols labile sulphide per mol enzyme. Ferricyanide was used as electron acceptor. The optimal reaction temperature was 85 °C under the assay conditions used and the pH optimum of the enzyme reaction was 8·0. The K m values for AMP and ferricyanide were 1 mm and 0·4 mm, respectively. The p1 of the archaebacterial adenylylsulphate reductase was 4·8., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-6-1419