SUMMARY: The rumen anaerobic fungi and were grown in the presence of cellulosic substrate (sisal fibres) and the properties of the glycosidases and polysaccharide-degrading enzymes produced by the organisms were studied. β--Glucosidase (EC, β--fucosidase (EC, β--galactosidase (EC, β-1,3-glucanase (EC, β-1,4-glucanase (EC and β-xylanase (EC had pH optima of 6.0 and temperature optima under the conditions of assay of 50-55 °C, whereas for β-xylosidase (EC the optima were 6.5 and 39 °C respectively. The apparent values of individual enzymes secreted by the three fungi were similar. The results show that and produce the same range of enzymes, with similar properties, able to degrade cellulose and hemicellulose.


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