%0 Journal Article %A Hébraud, Michel %A Fèvre, Michel %T Characterization of Glycoside and Polysaccharide Hydrolases Secreted by the Rumen Anaerobic Fungi Neocallimastix frontalis, Sphaeromonas communis and Piromonas communis %D 1988 %J Microbiology, %V 134 %N 5 %P 1123-1129 %@ 1465-2080 %R https://doi.org/10.1099/00221287-134-5-1123 %I Microbiology Society, %X The rumen anaerobic fungi Neocallimastix frontalis, Sphaeromonas communis and Piromonas communis were grown in the presence of cellulosic substrate (sisal fibres) and the properties of the glycosidases and polysaccharide-degrading enzymes produced by the organisms were studied. β d-Glucosidase (EC 3.2.1.21), β d-fucosidase (EC 3.2.1.38), β d-galactosidase (EC 3.2.1.23), β1,3-glucanase (EC 3.2.1.6), β1,4-glucanase (EC 3.2.1.4) and βxylanase (EC 3.2.1.8) had pH optima of 6.0 and temperature optima under the conditions of assay of 50–55 °C, whereas for βxylosidase (EC 3.2.1.37) the optima were 6·5 and 39 °C respectively. The apparent K m values of individual enzymes secreted by the three fungi were similar. The results show that S. communis, P. communis and N. frontalis produce the same range of enzymes, with similar properties, able to degrade cellulose and hemicellulose. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-5-1123