SUMMARY: We have shown that the blood anticoagulant activity (BAA) secreted by (designated myxaline) is a heat-stable molecule: a high-molecular-mass extracellular fraction also with an apparent BAA is a thermolabile protease. This property allowed us to assay the BAA content in crude boiled culture supernatants and to study the conditions under which it is produced. Heat-stable BAA is strictly extracellular and its production is restricted to vegetative growth in Unlike the other extracellular proteins, its production is not affected by mutations that regulate secretion; mutations that modify the extracellular proteolytic activity do not modulate the amount of myxaline produced either. Several other species of and one other myxobacterial species produce a heat-stable BAA during vegetative growth.


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