Genetic Analysis in Streptomyces ambofaciens

TAMARA SMOKVINA; FRANÇOIS FRANCOU; MARIO LUZZATI

doi:10.1099/00221287-134-2-395

Genetic Analysis in Streptomyces ambofaciens

TAMARA SMOKVINA, FRANÇOIS FRANCOU and MARIO LUZZATI
First Published: 01 February 1988 https://doi.org/10.1099/00221287-134-2-395

Abstract

SUMMARY: A chromosomal linkage map of ten markers was established for Streptomyces ambofaciens by the four-factor cross method and allele-gradient analysis. Mutants were obtained by nitrous acid treatment as well as by UV mutagenesis. The fertility of crosses was enhanced over 100-fold by pSAM2, a plasmid present in some strains of S. ambofaciens, and over 1000-fold by the conjugative plasmid pIJ303.

Received: 09/06/1987
Revised: 14/09/1987
Published Online: 01/02/1988

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Microbiology

Volume 134, Issue 2

Research Article

Free

Genetic Analysis in Streptomyces ambofaciens

Abstract

Most read this month

Most cited this month Most Cited RSS feed

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Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin

CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies

Metals, minerals and microbes: geomicrobiology and bioremediation

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Quantification of biofilm structures by the novel computer program comstat

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